College of Life Sciences, Northwest University, Xi'an, 710069, China.
College of Medical Technology, Xi'an Medical University, Xi'an, 710021, China.
Eur J Protistol. 2020 Jun;74:125692. doi: 10.1016/j.ejop.2020.125692. Epub 2020 Mar 10.
The morphogenesis and molecular phylogeny of two soil hypotrich ciliates, Australocirrus australis and A. aspoecki, collected from Xi'an, Shaanxi Province, China, were investigated using protargol preparation. The main features of morphogenesis of A. australis are as follows: (1) the parental adoral zone of membranelles is retained completely in the proter; (2) parental cirri and the undulating membranes are involved in the formation of six primordial streaks; (3) multiple fragmentation of the third dorsal kinety anlage; (4) more than three dorsomarginal kineties are formed. An early divider of A. aspoecki was also found, and its morphogenetic events resemble those of other Australocirrus species. Furthermore, the SSU rDNA sequence of the Shaanxi populations of A. australis and A. aspoecki was provided. The molecular phylogeny indicates that A. aspoecki clusters together with A. rubrus and two populations of A. australis with full support, which are separated from the clade comprising the two populations of A. shii.
采用蛋白银染色法，对采自中国陕西西安的两种土壤纤毛虫，澳州游仆虫（Australocirrus australis）和 A. aspoecki 的形态发生和分子系统发育进行了研究。A. australis 的形态发生的主要特征如下：（1）前口纤毛带在Protosoma 中完全保留；（2）亲代纤毛和波动膜参与了 6 个原始条纹的形成；（3） 第三次腹棘毛基体多次断裂；（4）形成多于 3 条腹缘动基体。还发现了 A. aspoecki 的早期分裂体，其形态发生事件类似于其他澳州游仆虫物种。此外，还提供了来自陕西的 A. australis 和 A. aspoecki 种群的 SSU rDNA 序列。分子系统发育表明，A. aspoecki 与 A. rubrus 和具有完全支持的两个 A. australis 种群聚类在一起，与包括两个 A. shii 种群的分支分开。
