Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, 9000 Gent, Belgium; Laboratory of Dairy Research, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, 9000 Gent, Belgium.
Int J Food Microbiol. 2020 Jun 16;323:108586. doi: 10.1016/j.ijfoodmicro.2020.108586. Epub 2020 Mar 9.
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), a culture based alternative for microbial diversity studies, is an attractive tool to dereplicate large numbers of isolates to a smaller set of representatives for downstream characterization. In the present study, MALDI-TOF MS, combined with a database of reference spectra compiled in previous studies, was applied to identify 88 non-starter lactic acid bacteria (NSLAB) isolated from 18 samples of four different artisanal cheeses produced in the Island of Naxos, Greece, from raw sheep and goat milk without the addition of starters. Eighty-four isolates (95.5%) could be identified directly via MALDI-TOF MS. Lactobacillus brevis and Lactobacillus plantarum were the dominant species, followed by Lactococcus lactis, Leuconostoc mesenteroides Lactobacillus paracasei, Lactobacillus rhamnosus, Pediococcus pentosaceus and Enterococcus faecium. The remaining four isolates represented species present in the database; however, within-species diversity was insufficiently covered. Additionally, pheS sequencing was applied to confirm identification.
基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）是一种基于培养的微生物多样性研究替代方法，它是一种很有吸引力的工具，可以将大量的分离物去重复到更小的一组代表物，以便进行下游特征描述。在本研究中，MALDI-TOF MS 与先前研究中汇编的参考光谱数据库相结合，用于鉴定从希腊纳克索斯岛的四种不同手工奶酪的 18 个样本中分离的 88 株非发酵剂乳酸菌（NSLAB），这些奶酪是由未经发酵剂添加的生羊奶和山羊奶制成的。84 株分离株（95.5%）可直接通过 MALDI-TOF MS 进行鉴定。短乳杆菌和植物乳杆菌是主要的优势种，其次是乳球菌、肠膜明串珠菌、副干酪乳杆菌、鼠李糖乳杆菌、戊糖片球菌和屎肠球菌。其余 4 株分离株代表数据库中存在的种，但种内多样性覆盖不足。此外，pheS 测序也被应用于确认鉴定。
