Ahmadsah Lenny S F, Kim Eiseul, Jung Youn-Sik, Kim Hae-Yeong
Department of Food Science and Biotechnology & Institute of Life Sciences and Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
J Microbiol Biotechnol. 2018 Jan 28;28(1):32-39. doi: 10.4014/jmb.1705.05044.
Samples of (a fermentation starter) obtained from the upper part of Borneo Island were analyzed for their lactic acid bacteria (LAB) and fungal diversity using both a culture-independent method (PCR-DGGE) and culture-dependent methods (SDS-PAGE and MALDI-TOF MS). and were detected by all three methods. In addition, , and were detected by PCR-DGGE. In contrast, , and were detected only by the culture-dependent methods. Our results indicate that the culture-independent method can be used to determine whether multiple laru samples originated from the same manufacturing region; however, using the culture-independent and the two culture-dependent approaches in combination provides a more comprehensive overview of the laru microbiota.
从婆罗洲岛上部获取的（一种发酵剂）样本，使用非培养方法（PCR-DGGE）和培养方法（SDS-PAGE和MALDI-TOF MS）分析其乳酸菌（LAB）和真菌多样性。所有三种方法都检测到了 和 。此外，PCR-DGGE检测到了 、 和 。相比之下，只有培养方法检测到了 、 和 。我们的结果表明，非培养方法可用于确定多个laru样本是否源自同一生产区域；然而，将非培养方法与两种培养方法结合使用能更全面地了解laru微生物群。
