Division of Anaerobe Research, Life Science Research Center, Gifu University, Gifu, Japan; Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan.
Division of Anaerobe Research, Life Science Research Center, Gifu University, Gifu, Japan.
J Glob Antimicrob Resist. 2020 Jun;21:65-67. doi: 10.1016/j.jgar.2020.02.034. Epub 2020 Mar 19.
The aim of this study was to report the draft genome sequences of two multidrug-resistant bacteria (Bacteroides thetaiotaomicron F9-2 and Escherichia coli 09-02E) isolated from stool samples of a healthy resident in Vietnam.
Genome sequences were determined using MiSeq and MinION platforms. Genome assembly was performed using Platanus Assembler v.1.2.4 and Canu v.1.7. The DDBJ Fast Annotation and Submission Tool were used for genome annotation.
The genome of B. thetaiotaomicron F9-2 comprised 6 283 774 bp with a GC content of 42.7% and 4802 protein coding sequences (CDS), whereas the genome of E. coli 09-02E comprised 5 246 320 bp with a GC content of 50.6% and 4991 protein CDS. Both strains harboured common antimicrobial resistance genes, such as those for sulfonamides (sul2) and aminoglycosides (strA, strB). However, the sul2-strA-strB cassette was located on the chromosome of B. thetaiotaomicron F9-2, whereas it was located on a plasmid in E. coli 09-02E. These genes were flanked by different insertion sequences.
Considering their diversities in the human gut resistome, these strains would be of considerable interest for detailed comparative genomic analysis. Notably, the same sul2 cassette was found in facultative and obligate anaerobic bacterial isolates (resident in humans). However, the different location of the cassette indicates a possible mechanism of gene transfer among gut microbes.
本研究旨在报告从越南一名健康居民粪便样本中分离出的两种多药耐药菌（拟杆菌 F9-2 和大肠杆菌 09-02E）的基因组序列草案。
使用 MiSeq 和 MinION 平台测定基因组序列。使用 Platanus 组装程序 v.1.2.4 和 Canu v.1.7 进行基因组组装。使用 DDBJ Fast Annotation and Submission Tool 进行基因组注释。
拟杆菌 F9-2 的基因组包含 6283774bp，GC 含量为 42.7%，有 4802 个蛋白编码序列（CDS）；大肠杆菌 09-02E 的基因组包含 5246320bp，GC 含量为 50.6%，有 4991 个蛋白 CDS。两种菌株均携带常见的抗生素耐药基因，如磺胺类（sul2）和氨基糖苷类（strA、strB）。然而，sul2-strA-strB 盒位于拟杆菌 F9-2 的染色体上，而在大肠杆菌 09-02E 中位于质粒上。这些基因被不同的插入序列所包围。
考虑到它们在人类肠道耐药组中的多样性，这些菌株将非常适合进行详细的比较基因组分析。值得注意的是，同一个 sul2 盒在兼性和专性厌氧菌分离株（驻留在人类体内）中都有发现。然而，盒的不同位置表明了肠道微生物之间基因转移的可能机制。
