Department of Infection Control and Prevention, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan.
Department of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, Japan.
J Glob Antimicrob Resist. 2019 Sep;18:148-150. doi: 10.1016/j.jgar.2019.07.001. Epub 2019 Jul 8.
The emergence and spread of carbapenemase-producing Enterobacteriaceae is a worldwide concern. This study reports the whole genome sequence of an NDM-5-, CTX-M-14-, OXA-10- and MCR-1-co-producing Escherichia coli sequence type 167 (ST167) multidrug-resistant clinical strain (EC129) isolated from a sputum sample of a hospitalised patient diagnosed with pneumonia.
The genome of E. coli EC129 was subjected to next-generation sequencing and reads were assembled. The draft genome was annotated using DDBJ Read Annotation Pipeline DFAST server, followed by subsequent in silico analysis.
The genome of E. coli ST167 strain EC129 is 5319159 bp in length and contains 5022 protein-coding sequences. The bla, bla, bla and mcr-1 genes were detected along with other antimicrobial resistance genes conferring resistance to aminoglycosides, fluoroquinolones, sulfonamides, trimethoprim and tetracyclines. Antimicrobial susceptibility testing revealed that the isolate was resistant to all antimicrobial agents except colistin.
To our knowledge, this study is the first to report anE. coli ST167 strain co-producing NDM-5, CTX-M-14, OXA-10 and MCR-1 isolated from a sputum sample of an individual with pneumonia in Japan, thus elucidating the molecular characteristics and resistance gene diversity of this strain.
产碳青霉烯酶肠杆菌科的出现和传播是一个全球性的问题。本研究报告了一株同时产 NDM-5、CTX-M-14、OXA-10 和 MCR-1 的携带型大肠埃希氏菌（ST167）多重耐药临床株（EC129）的全基因组序列，该菌从一名诊断为肺炎的住院患者的痰样本中分离得到。
对大肠埃希氏菌 EC129 的基因组进行了下一代测序，并对读取进行了组装。使用 DDBJ Read Annotation Pipeline DFAST 服务器对基因组草案进行注释，然后进行后续的计算机分析。
ST167 型大肠埃希氏菌 EC129 基因组全长 5319159 bp，包含 5022 个蛋白编码序列。检测到 bla、bla、bla 和 mcr-1 基因以及其他赋予对氨基糖苷类、氟喹诺酮类、磺胺类、甲氧苄啶和四环素类药物耐药性的抗生素耐药基因。药敏试验显示，该分离株除对黏菌素外，对所有抗菌药物均有耐药性。
据我们所知，本研究首次报告了一株产 NDM-5、CTX-M-14、OXA-10 和 MCR-1 的肺炎患者痰样本中分离的大肠埃希氏菌 ST167 株，阐明了该株的分子特征和耐药基因多样性。
