Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran; Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
J Chromatogr A. 2020 Jun 21;1621:461045. doi: 10.1016/j.chroma.2020.461045. Epub 2020 Mar 13.
New zwitterionic (ZIC) stationary phases (SPs) are synthesized with the click and conventional bonding of tyrosine to silica gel. Infrared spectra and elemental analysis demonstrate the successful click and conventional bonding of this ZIC group on silica particles by the surface coverage including 2.36 and 0.75 µm m, respectively. Given the above-mentioned explanation, the present study evaluated the retention mechanism and chromatographic manners of polar compounds on these new materials under hydrophilic interaction liquid chromatography (HILIC) conditions. Based on the results, the Click-Tyrosine Stationary Phase provided good HILIC characteristics when it was applied to separate phenolic compounds, amino acids, alkaloids, and nucleobases compared to bare silica gel SP and even conventional tyrosine SPs. Further, this new Click-Tyrosine-SP represented appropriate HILIC features and column efficiency (the theoretical plate number was up to 50,000 plates m for thebaine). Furthermore, the study investigated the effect of solute polarity (the number of the hydroxyl group of phenolic compounds) and hydrophobicity (the number of the side chain of aliphatic amino acids) on retention behaviors. Finally, some important factors were studied as the potential variables for guiding the retention behavior of the polar compound in HILIC condition including solvent composition, salt concentration, and the buffer pH of the mobile phase.
新型两性离子（ZIC）固定相（SP）通过点击和酪氨酸与硅胶的常规键合合成。红外光谱和元素分析表明，通过表面覆盖率，成功地将 ZIC 基团通过点击和常规键合键合到硅胶颗粒上，分别为 2.36 和 0.75 μm m。基于上述解释，本研究在亲水相互作用液相色谱（HILIC）条件下评估了极性化合物在这些新材料上的保留机制和色谱方式。基于结果，与裸硅胶 SP 甚至常规酪氨酸 SP 相比，Click-Tyrosine 固定相在分离酚类化合物、氨基酸、生物碱和核苷时提供了良好的 HILIC 特性。此外，这种新型 Click-Tyrosine-SP 表现出适当的 HILIC 特性和柱效率（thebaine 的理论塔板数高达 50,000 块 m）。此外，该研究还研究了溶质极性（酚类化合物的羟基数量）和疏水性（脂肪族氨基酸的侧链数量）对保留行为的影响。最后，研究了一些重要因素，作为指导极性化合物在 HILIC 条件下保留行为的潜在变量，包括溶剂组成、盐浓度和流动相的缓冲 pH 值。
