一种用于亲水相互作用液相色谱的新型点击赖氨酸两性离子固定相。
A novel click lysine zwitterionic stationary phase for hydrophilic interaction liquid chromatography.
机构信息
School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
出版信息
J Chromatogr A. 2012 Feb 3;1223:47-52. doi: 10.1016/j.chroma.2011.12.033. Epub 2011 Dec 26.
A novel type of zwitterionic HILIC stationary phase was prepared by covalently bonding the l-azido lysine on silica gel via click chemistry. The key intermediate azido lysine was synthesized by transformation the amino group in l-Boc-lysine to corresponding azido group and subsequent removal of the N-protected group (Boc). Finally, the azido lysine was covalently bonded to silica beads by click chemistry to get click lysine. Its structure was confirmed by FT-IR and elemental analysis. The new stationary phase showed good HILIC characteristics, when it was applied to separate polar and hydrophilic compounds, such as organic acids, cephalosporins and carbapenems. Compared with the commercial stationary phases, such as Atlantics HILIC and ZIC-HILIC, click lysine displayed better or similar chromatographic behaviors.
通过点击化学，将 l-叠氮赖氨酸通过共价键连接到硅胶上，制备了一种新型两性离子亲水作用色谱固定相。关键中间体叠氮赖氨酸是通过将 l-Boc-赖氨酸中的氨基转化为相应的叠氮基团，然后去除 N-保护基（Boc）来合成的。最后，通过点击化学将叠氮赖氨酸共价键合到硅胶珠上得到点击赖氨酸。其结构通过傅里叶变换红外光谱（FT-IR）和元素分析得到确认。当该新型固定相应用于分离极性和亲水性化合物，如有机酸、头孢菌素和碳青霉烯类时，表现出良好的亲水作用色谱特性。与商业固定相（如 Atlantics HILIC 和 ZIC-HILIC）相比，点击赖氨酸显示出更好或相似的色谱行为。
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