Bioproduct Engineering, University of Groningen, Nijenborgh 4, Groningen, 9747 AG, the Netherlands.
Anal Biochem. 2020 May 15;597:113696. doi: 10.1016/j.ab.2020.113696. Epub 2020 Mar 19.
Amylolytic enzymes are a group of proteins degrading starch to its constitutional units. For high-throughput screening, simple yet accurate methods in addition to the reducing ends assays are required. In this article, the iodine assay, a photometric assay based on the intensely colored starch-iodine complex, was adapted to enable accurate and objective differentiation between enzyme and background activity using a newly introduced mathematical factor. The method was further improved by designing a simple setup for multiple time point detection and discussing the applicability of single wavelength measurements.
淀粉分解酶是一组将淀粉降解为其组成单位的蛋白质。为了实现高通量筛选，除了还原末端测定法之外，还需要简单而准确的方法。本文中，碘量法，一种基于强烈显色的淀粉-碘复合物的比色法，通过引入一个新的数学因子，被改编以实现酶活性和背景活性的准确和客观区分。该方法通过设计一个用于多点检测的简单装置进一步得到改进，并讨论了单波长测量的适用性。
