Biochemistry and Immunology, University Hospital of Southern Denmark, Vejle, Denmark.
Biochemistry and Immunology, University Hospital of Southern Denmark, Vejle, Denmark.
Clin Biochem. 2020 Aug;82:51-57. doi: 10.1016/j.clinbiochem.2020.03.006. Epub 2020 Mar 19.
The catecholamines, epinephrine (E) and norepinephrine (NE) are important both as neurotransmitters and hormones, and measurement of E and NE in plasma is therefore of great interest in medical research. However, the low concentrations of E and NE in plasma require an analysis method that is both specific and sensitive. Plasma sample E and NE were extracted using solid phase extraction, and analyzed by an in-sample ion-pairing chromatography (IPC) LC-MS/MS method, that enables the ion-pairing reagent to be diverted to waste without entering the ion source of the mass spectrometer. The method was validated with good performance characteristics and the limit of quantification (LOQ) was found to be 0.20 and 0.02 nmol/L for NE and E, respectively. In conclusion, this work presents development and validation of a method for determining E and NE in plasma with a measuring range covering the entire reference interval for human plasma.
儿茶酚胺，肾上腺素（E）和去甲肾上腺素（NE）既是神经递质又是激素，因此血浆中 E 和 NE 的测量在医学研究中非常重要。然而，血浆中 E 和 NE 的低浓度需要一种既特异又灵敏的分析方法。使用固相萃取法提取血浆样本中的 E 和 NE，并用样品内离子对色谱（IPC）LC-MS/MS 法进行分析，该方法可使离子对试剂在不进入质谱仪离子源的情况下被转移到废物中。该方法具有良好的性能特征，NE 和 E 的定量限（LOQ）分别为 0.20 和 0.02 nmol/L。总之，本工作提出了一种用于测定血浆中 E 和 NE 的方法的开发和验证，该方法的测定范围涵盖了人血浆的整个参考区间。
