Cichocki T, Heck D, Jarczyk L, Rokita E, Strzałkowski A, Sych M
Department of Histology, Academy of Medicine, Cracow, Poland.
Folia Histochem Cytobiol. 1988;26(4):187-92.
Inorganic deposits in the wall of human and animal arteries and in experimental tumor (Morris hepatoma 7777) were examined using proton induced X-ray emission (PIXE) and PIXE in combination with proton microprobe (micro-PIXE) techniques. The sections adjacent to the irradiated ones part were submitted to histological investigations and one part of the material was additionally investigated by infrared (IR) spectroscopy. For identification of mineral deposits, the micro-PIXE method appeared the most sensitive. The mineral deposits were detected in the artery samples, even in those without visible morphological changes, as well as in tumor samples. The deposites showed different localization and composition, depending on age and type of vessel. There were also differences between human and animal arteries. IR spectroscopy revealed the presence of carbonate apatite within the artery samples from old individuals. Matching of histological observations with data obtained by micro-PIXE method allows a better correlation of morphological and analytical results.
使用质子激发X射线发射（PIXE）以及PIXE与质子微探针（显微PIXE）相结合的技术，对人和动物动脉壁以及实验性肿瘤（莫里斯肝癌7777）中的无机沉积物进行了检测。将与照射部位相邻的切片进行组织学研究，并且一部分材料还通过红外（IR）光谱进行了额外研究。对于矿物沉积物的识别，显微PIXE方法似乎最为灵敏。在动脉样本中检测到了矿物沉积物，即使在那些没有明显形态变化的样本中，以及在肿瘤样本中也检测到了。这些沉积物根据血管的年龄和类型呈现出不同的定位和组成。人和动物的动脉之间也存在差异。红外光谱显示，老年个体的动脉样本中存在碳酸磷灰石。将组织学观察结果与通过显微PIXE方法获得的数据相匹配，能够更好地关联形态学和分析结果。
