Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, P. R. China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shanxi 712100, P. R. China.
Environ Sci Technol. 2020 Apr 21;54(8):4932-4941. doi: 10.1021/acs.est.0c00359. Epub 2020 Apr 2.
This study investigated the tissue-specific accumulation and biotransformation of 6:6 and 8:8 perfluoroalkyl phosphinic acids (PFPiA) in common carp () during 90 d exposure and 30 d depuration in water in the laboratory. Both 6:6 and 8:8 PFPiAs could quickly accumulate in the carp, and 6:6 PFPiA displayed higher bioaccumulation potential than 8:8 PFPiA. The highest concentrations of PFPiAs were observed in the blood, while the lowest were found in the muscle. The equilibrium dialysis experiment indicated that both PFPiAs had higher binding affinities with the proteins in the fish serum than in liver, which was supported by the molecular docking analysis. The results also indicated that 6:6 PFPiA had higher binding affinities with the serum and liver proteins than 8:8 PFPiA. These results suggested that the tissue-specific distribution of PFPiAs was highly dependent on the binding affinities with the specific proteins. Both and experiments consistently indicated that PFPiAs experienced biotransformation and produced perfluoroalkyl phosphonic acids (PFPAs), and biotransformation of 8:8 PFPiA was more active than 6:6 PFPiA. It was worth noting that perfluorohexanonate and perfluorooctanoic acids were identified in fish as metabolites after long-term exposure to PFPiAs for the first time.
本研究在实验室中研究了 6:6 和 8:8 全氟烷基亚磷酸（PFPiA）在鲤鱼（） 90 天暴露和 30 天水净化期间的组织特异性积累和生物转化。6:6 和 8:8 PFPiA 均可迅速在鲤鱼体内积累，且 6:6 PFPiA 的生物积累潜力高于 8:8 PFPiA。PFPiA 的最高浓度出现在血液中，而最低浓度出现在肌肉中。平衡透析实验表明，两种 PFPiA 与鱼类血清中的蛋白质具有更高的结合亲和力，而与肝脏中的蛋白质结合亲和力较低，分子对接分析支持了这一结果。结果还表明，6:6 PFPiA 与血清和肝脏蛋白质的结合亲和力均高于 8:8 PFPiA。这些结果表明，PFPiA 的组织特异性分布高度依赖于与特定蛋白质的结合亲和力。和 实验均一致表明，PFPiA 经历了生物转化并产生了全氟烷基亚磷酸（PFPAs），并且 8:8 PFPiA 的生物转化比 6:6 PFPiA 更活跃。值得注意的是，首次在长期暴露于 PFPiA 的鱼类中发现了全氟己烷和全氟辛酸作为代谢物。
