Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, China; Anhui Province Key Laboratory of Major Autoimmune Diseases, 81 Meishan Road, Hefei, Anhui, China.
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, China; Anhui Province Key Laboratory of Major Autoimmune Diseases, 81 Meishan Road, Hefei, Anhui, China.
Arch Oral Biol. 2020 May;113:104708. doi: 10.1016/j.archoralbio.2020.104708. Epub 2020 Mar 14.
The important role of intestinal microbiota in systemic lupus erythematosus (SLE) has been recognized. Oral-gut microbiome axis is a crucial link in human health and disease, but few researches indicated the relationship between oral microorganisms and SLE. This study mainly explored the composition and changes of oral microorganisms in SLE patients with different stages, clinical manifestations and biomarkers.
Oral microbiota was detected by 16S ribosomal RNA gene sequencing from 20 SLE patients and 19 healthy controls (HCs). The evenness, diversity and composition of oral microbiota were analyzed. Moreover, receiver-operating characteristic analysis was conducted. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) based on Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to investigate microbiota functions.
The oral microbiota of SLE patients was imbalanced and the diversity was decreased, but no difference was found between new-onset and treated SLE patients. Families Lactobacillaceae, Veillonellaceae and Moraxellaceae were enriched in SLE patients. Families like Corynebacteriaceae, Micrococcaceae, Defluviitaleaceae, Caulobacteraceae, Phyllobacteriaceae, Methylobacteriaceae, Hyphomicrobiaceae, Sphingomonadaceae, Halomonadaceae, Pseudomonadaceae, Xanthomonadaceae, etc. were decreased in SLE patients. After multiple testing adjustment, families Sphingomonadaceae, Halomonadaceae, and Xanthomonadaceae were significantly decreased in SLE patients. And area under the curve was 0.953 (95% confidence intervals 0.890-1.000) to distinguish SLE patients from HCs. There were differences in metabolic pathways between SLE and HCs (P = 0.025).
These findings collectively support that oral microbiota dysbiosis and aberrant metabolic pathways were observed in patients with SLE. Our findings may provide suggestive evidences for the diagnosis and treatment of SLE.
肠道微生物群在系统性红斑狼疮（SLE）中的重要作用已得到认可。口腔-肠道微生物轴是人类健康和疾病的关键环节，但很少有研究表明口腔微生物与 SLE 之间的关系。本研究主要探讨了不同阶段、临床表现和生物标志物的 SLE 患者口腔微生物的组成和变化。
通过 16S 核糖体 RNA 基因测序从 20 名 SLE 患者和 19 名健康对照者（HCs）中检测口腔微生物群。分析口腔微生物群落的均匀度、多样性和组成。此外，进行了接收者操作特征分析。基于京都基因与基因组百科全书（KEGG）数据库的群落重建未观察状态的系统发育分析（PICRUSt）用于研究微生物群功能。
SLE 患者的口腔微生物群落失衡，多样性降低，但新发和治疗的 SLE 患者之间无差异。乳杆菌科、韦荣球菌科和莫拉氏菌科在 SLE 患者中富集。棒状杆菌科、微球菌科、脱卤杆菌科、柄杆菌科、叶杆菌科、甲基杆菌科、噬氢菌科、鞘氨醇单胞菌科、盐单胞菌科、假单胞菌科、黄单胞菌科等家族在 SLE 患者中减少。经过多次测试调整，鞘氨醇单胞菌科、盐单胞菌科和黄单胞菌科在 SLE 患者中显著减少。区分 SLE 患者和 HCs 的曲线下面积为 0.953（95%置信区间 0.890-1.000）。SLE 与 HCs 之间存在代谢途径的差异（P=0.025）。
这些发现共同支持在 SLE 患者中观察到口腔微生物群落失调和异常代谢途径。我们的发现可能为 SLE 的诊断和治疗提供提示性证据。
