State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin 150001, China.
State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Harbin 150001, China.
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110742. doi: 10.1016/j.msec.2020.110742. Epub 2020 Feb 12.
To delay the degradation of magnesium alloys, silk fibroin as a natural organic polymer coating was fabricated on a 3-amino-propyltriethoxysilane (APTES) pretreated Mg-Zn-Ca alloy. APTES pretreatment coated the surface of magnesium alloys with amino groups, which can bond with functional groups in silk fibroin to form a compact coating/substrate interface. The influences of the APTES concentration and drying temperature on the coating adhesion and interface were investigated to explore the optimal parameters in the fabrication process. The nanoporous silk fibroin films completely covered the APTES pretreated Mg-Zn-Ca surface, which reached a thickness of ~7 μm. The chemical states for the coated Mg-Zn-Ca alloy were compared to those of the bare Mg-Zn-Ca alloy and the APTES pretreated Mg-Zn-Ca alloy to illustrate the coating mechanism. During in vitro degradation and electrochemical measurements in simulated body fluid (SBF), the samples with the silk fibroin coating showed remarkably improved corrosion resistance and a slower degradation rate compared to those of the bare samples, suggesting that the silk fibroin coating was an effective protection coating for the substrates and can delay the degradation of magnesium alloys. Moreover, a model for the in vitro degradation was proposed. In vitro cell experiments confirmed the excellent biocompatibility of silk fibroin coated Mg-Zn-Ca structure.
为了延缓镁合金的降解，在经过 3-氨丙基三乙氧基硅烷（APTES）预处理的 Mg-Zn-Ca 合金上制备了丝素蛋白作为天然有机聚合物涂层。APTES 预处理在镁合金表面涂覆了氨基，这些氨基可以与丝素蛋白的官能团结合，在涂层/基底界面形成致密的结合。研究了 APTES 浓度和干燥温度对涂层附着力和界面的影响，以探索制备过程中的最佳参数。纳米多孔丝素蛋白薄膜完全覆盖了 APTES 预处理的 Mg-Zn-Ca 表面，厚度达到~7 μm。对涂层的 Mg-Zn-Ca 合金进行化学状态分析，与裸 Mg-Zn-Ca 合金和 APTES 预处理的 Mg-Zn-Ca 合金进行比较，以说明涂层的形成机制。在模拟体液（SBF）中的体外降解和电化学测量中，与裸样相比，具有丝素蛋白涂层的样品表现出显著提高的耐腐蚀性和更慢的降解速率，这表明丝素蛋白涂层是基底的有效保护涂层，可以延缓镁合金的降解。此外，还提出了一个体外降解模型。体外细胞实验证实了丝素蛋白涂层 Mg-Zn-Ca 结构具有优异的生物相容性。
