[一种新型的A血型和O（H）血型抗原——来自猪红细胞的糖鞘脂]

[A novel blood group A- and O (H) -antigens--glycosphingolipids from porcine erythrocytes].

作者信息

Sako F

机构信息

Department of Home Economics, Hokkaido University of Education, Sapporo, Japan.

出版信息

Hokkaido Igaku Zasshi. 1988 Jul;63(4):534-44.

PMID:3220436

Abstract

Five neutral glycosphingolipids (GSLs) (Gb4Cer, Gb3Cer, LacCer, GlcCer and GalCer) were isolated from porcine erythrocyte membranes. Gb4Cer was the most abundant (over 70% of total neutral GSLs). In addition, a minor GSL fraction (less than 1% of the total), which migrated more slowly than Gb5Cer on TLC, composed of five GSLs. Two of them exhibited blood group A and O (H) activity, respectively, and were studied for their structures by spectrometries with NMR and FAB-mass, sequential hydrolysis by exoglycosidases, methylation and immunostaining. Chemical structure of the blood group A-antigen was proposed to be GalNAc alpha 1-3 (Fuc alpha 1-2) Gal beta 1-3 Gal-4Glc beta 1NAc beta 1-3 Gal beta 1-4 Glc beta 1-1Cer and GalNAc alpha 1-3 (Fuc alpha 1-2) Gal beta 1-4 GlcNAc beta 1-3 Gal beta 1-4Glc beta 1-1Cer. The blood group O (H)-antigen showed the structure lacking non-reducing terminal alpha-linked N-acetylgalactosamine from the A-antigen, or a biosynthetic precursor from of the A-antigen.

摘要

从猪红细胞膜中分离出五种中性糖鞘脂（GSLs）（Gb4Cer、Gb3Cer、乳糖神经酰胺、葡萄糖神经酰胺和半乳糖神经酰胺）。Gb4Cer含量最高（占总中性GSLs的70%以上）。此外，还有一小部分GSL（占总量不到1%），在薄层层析（TLC）上迁移速度比Gb5Cer慢，由五种GSL组成。其中两种分别表现出血型A和O（H）活性，并通过核磁共振（NMR）光谱、快原子轰击质谱（FAB - mass）、外切糖苷酶顺序水解、甲基化和免疫染色对其结构进行了研究。血型A抗原的化学结构被认为是GalNAcα1 - 3（Fucα1 - 2）Galβ1 - 3 Gal - 4Glcβ1NAcβ1 - 3 Galβ1 - 4 Glcβ1 - 1Cer和GalNAcα1 - 3（Fucα1 - 2）Galβ1 - 4 GlcNAcβ1 - 3 Galβ1 - 4Glcβ1 - 1Cer。血型O（H）抗原的结构显示，其缺乏A抗原非还原末端的α连接N - 乙酰半乳糖胺，或是A抗原的生物合成前体。