Makita A, Gasa S, Sako F, Hanagata G
Biochemistry Laboratory, Hokkaido University School of Medicine, Sapporo, Japan.
Indian J Biochem Biophys. 1990 Dec;27(6):402-10.
Glycosphingolipids were purified from porcine erythrocytes and plasma. Two minor glycolipids with human blood group A and H antigenicities were found in both sources as components. The two antigenic glycolipids were identified as a hexaglycosylceramide (IV3 alpha GalNAc,IV2 alpha Fuc-Lc4Cer) for the A antigen and pentaglycosylceramide (IV2 alpha Fuc-Lc4Cer) for the H antigen and belonged to lactoseries (type 1 sugar chain) in contrast to those with neolacto core (type 2 sugar chain) in human erythrocytes, thereby endorsing biochemically the previous serological observations that the A antigen on porcine erythrocytes is uptake from plasma, probably the H antigen being the case. In addition to major glycolipids of globoseries in red cells and plasma, a variety of acidic glycolipids including two classes of sulphatides (sulphated galactosylceramide and sulphated lactosylceramide) and five classes of gangliosides (GM3, GD3, GM1, fucosyl GM1 and GD1a) containing N-acetylneuraminic acid and N-glycolylneuraminic acid were obtained from plasma.
从猪红细胞和血浆中纯化糖鞘脂。在这两种来源中均发现两种具有人血型A和H抗原性的次要糖脂作为成分。这两种抗原性糖脂被鉴定为A抗原的六糖基神经酰胺（IV3αGalNAc，IV2αFuc-Lc4Cer）和H抗原的五糖基神经酰胺（IV2αFuc-Lc4Cer），它们属于乳糖系列（1型糖链），与人类红细胞中具有新乳糖核心（2型糖链）的糖脂不同，从而从生化角度证实了先前的血清学观察结果，即猪红细胞上的A抗原是从血浆中摄取的，H抗原可能也是如此。除了红细胞和血浆中主要的球系列糖脂外，还从血浆中获得了多种酸性糖脂，包括两类硫脂（硫酸化半乳糖基神经酰胺和硫酸化乳糖基神经酰胺）和五类神经节苷脂（GM3、GD3、GM1、岩藻糖基GM1和GD1a），它们含有N-乙酰神经氨酸和N-糖基神经氨酸。
