Moghtaderi Arezo, Raji Ahmadreza, Khanzadi Saied, Nabipour Abolghasem
DVSC Graduate, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Vet Res Forum. 2019 Fall;10(4):357-360. doi: 10.30466/vrf.2018.89154.2160. Epub 2019 Dec 15.
Nowadays, the consumption of meat and meat products has been increased with modern manufacturing techniques. Due to the economic value, the likelihood of using unauthorized tissue is possible in meat products. The aim of this study was to apply morphological methods for detection of unauthorized tissues in meat sausage. In this study, a total number of 20 samples of different types of sausages were randomly collected from markets, in north-east of Iran. Each sample was divided into three equal parts and three paraffin-embedded blocks were prepared from each part (180 blocks). Then the sections were stained using Hematoxylin and Eosin, Masson's trichrome, Periodic acid- Schiff/Alcian blue and Verhoeffe/Van Gieson. A total number of 720 slides were observed using a light microscope. This research showed the use of unauthorized tissues in the sausages which was detected by histological methods. We observed authorized tissues like skeletal muscle fiber (100%), fat tissue (100%) and plant material (97.70%). A wide range of unauthorized tissues were detected including dense connective tissue (6.66%), cartilage (28.30%), bone (8.30%), skin (51.60%), smooth muscle (1.66%) and blood vessels (11.66%). The results of this study confirmed the use of unauthorized tissue in meat sausages in Iran and concluded that the histological methods especially Masson's trichrome staining are a practical technique for routine assessment of authenticity and quality of sausage to protect the consumers from adulteration.
如今，随着现代制造技术的发展，肉类和肉制品的消费量有所增加。由于经济价值的原因，肉制品中使用未经授权组织的可能性是存在的。本研究的目的是应用形态学方法检测肉肠中未经授权的组织。在本研究中，从伊朗东北部的市场随机收集了总共20个不同类型香肠的样本。每个样本被分成三个相等的部分，每个部分制备三个石蜡包埋块（共180个块）。然后使用苏木精和伊红、Masson三色染色法、过碘酸-希夫/阿尔辛蓝染色法以及Verhoeffe/Van Gieson染色法对切片进行染色。使用光学显微镜观察了总共720张载玻片。这项研究表明通过组织学方法检测到香肠中使用了未经授权的组织。我们观察到了如骨骼肌纤维（100%）、脂肪组织（100%）和植物材料（97.70%）等授权组织。还检测到了多种未经授权的组织，包括致密结缔组织（6.66%）、软骨（28.30%）、骨（8.30%）、皮肤（51.60%）、平滑肌（1.66%）和血管（11.66%）。本研究结果证实了伊朗肉肠中存在使用未经授权组织的情况，并得出结论，组织学方法尤其是Masson三色染色法是一种用于常规评估香肠真伪和质量的实用技术，可保护消费者免受掺假的影响。
