Esmaili Nejad Mohammad Reza, Vafaei Rana, Masoudifard Majid, Nassiri Seyed Mahdi, Salimi Atena
Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Vet Res Forum. 2019 Fall;10(4):361-364. doi: 10.30466/vrf.2019.100779.2402. Epub 2019 Dec 15.
Canine osteosarcoma (OS) or osteogenic sarcoma is an aggressive tumor of the skeletal system, associated with a rapid progression and guarded prognosis. The osteosarcomas, mostly arise from the appendicular skeleton while axial OS (osteosarcoma of flat bones) are less reported in the majority of large breeds. This report describes complete para-clinical investigations of an aggressive chondroblastic OS involving facial flat bones with highly metastatic characterization in a large mix breed stray dog. Radiographic and computed tomography findings demonstrated an amorphous and active new bone formation, associated with the severe lytic areas in the left maxillary, orbital and zygomatic bones. Also, lots of nodular densities were distributed in all lung lobes. The cytological examination of the mass revealed individualized oval to spindle-shaped pleomorphic mesenchymal cells exhibiting many criteria of malignancy such as marked anisocytosis, anisokaryosis, prominent and multiple nucleoli. The punctate cytoplasmic vacuoles were obvious and bi-nucleated cells were frequently observed. These cells were seen in the background of an eosinophilic matrix. Histopathologic evaluation of the mass exhibited areas of osseous differentiation within the mass containing bony spicules and wavy bone formation along with the vast areas of cartilaginous differentiations with chondroblasts in lacunar spaces. Ultimately, chondroblastic OS with severe lung metastasis was confirmed and the animal was euthanized.
犬骨肉瘤（OS）或成骨肉瘤是一种侵袭性骨骼系统肿瘤，进展迅速且预后不佳。骨肉瘤大多起源于四肢骨骼，而轴性骨肉瘤（扁骨骨肉瘤）在大多数大型犬种中报道较少。本报告描述了一只大型混种流浪犬面部扁骨发生的具有高度转移特征的侵袭性软骨母细胞性骨肉瘤的完整临床旁检查。X线和计算机断层扫描结果显示有不规则的活跃新骨形成，伴有左上颌骨、眶骨和颧骨的严重溶骨区。此外，所有肺叶均分布有许多结节状密度影。肿块的细胞学检查显示为单个的椭圆形至梭形多形性间充质细胞，表现出许多恶性标准，如明显的细胞大小不等、核大小不等、显著且多个核仁。点状细胞质空泡明显，双核细胞常见。这些细胞见于嗜酸性基质背景中。肿块的组织病理学评估显示肿块内有骨分化区域，包含骨小梁和波浪状骨形成，同时还有大片软骨分化区域，软骨细胞位于陷窝内。最终，确诊为伴有严重肺转移的软骨母细胞性骨肉瘤，该动物被安乐死。
