Barçante T A, Barçante J M P, Cardoso L, Remedio R N, Lima W S
Department of Health Sciences, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil.
Department of Parasitology, Federal University of Minas Gerais, Minas Gerais, Brazil.
J Parasitol Res. 2020 Mar 5;2020:4627158. doi: 10.1155/2020/4627158. eCollection 2020.
The intermediate hosts of the French heartworm are aquatic and terrestrial gastropods. The present work is aimed at clarifying the sites of penetration and the migratory routes of in snail tissues and evaluating their perilarval reaction with regard to the cellular composition and histological alterations involved in the gastropod response to infection. snails were individually infected with 1000 first-stage larvae (L1) of each and killed at predetermined times after infection. Percutaneous infection occurred simultaneously with oral infection. Despite larval tropism to the fibromuscular tissue, some larvae were located in different tissues and organs. A perilarval reaction was observed around the larvae in a fibromuscular layer, appearing later around the larvae located in the viscera. The number of hemocytes surrounding the larvae increased gradually, forming a pregranuloma. Larval death and degeneration were not observed. No defined migratory pattern occurred, and larval development was apparently not associated with particular tissues or organs. In addition, the infection by induces a systemic mobilization of hemocytes in perilarval reaction.
法国心丝虫的中间宿主是水生和陆生腹足纲动物。本研究旨在阐明其在蜗牛组织中的侵入部位和迁移途径，并根据腹足纲动物对感染的细胞组成和组织学改变，评估其围幼虫反应。将蜗牛分别感染1000条每种寄生虫的第一期幼虫（L1），并在感染后的预定时间处死。经皮感染与经口感染同时发生。尽管幼虫具有向纤维肌肉组织的趋向性，但一些幼虫位于不同的组织和器官中。在纤维肌肉层的幼虫周围观察到围幼虫反应，在内脏中的幼虫周围出现得较晚。围绕幼虫的血细胞数量逐渐增加，形成前肉芽肿。未观察到幼虫死亡和退化。未出现明确的迁移模式，幼虫发育显然与特定组织或器官无关。此外，该寄生虫感染在围幼虫反应中诱导血细胞的全身性动员。
