Le Harzic Ronan, Meiser Ina, Neubauer Julia C, Riemann Iris, Schiffer Michael, Stracke Frank, Zimmermann Heiko
Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany.
Fraunhofer Project Centre for Stem Cell Process Engineering, Neunerplatz 2, 97082 Würzburg, Germany.
Biomed Opt Express. 2020 Jan 2;11(2):517-532. doi: 10.1364/BOE.11.000517. eCollection 2020 Feb 1.
We present a novel optical device developed for the monitoring of dynamic behavior in extended 3D-tissue models in various culture environments based on variations in their speckle patterns. The results presented point out the benefit of the technology in terms of detection, accuracy, sensitivity and a reasonable read-out speed as well as reproducibility for the measurements and monitoring of cardiac contractions. We show that the optical read-out technology is suitable for long time monitoring and for drug screening. The method is discussed and compared to other techniques, in particular calcium imaging. The device is flexible and easily adaptable to 2D and 3D-tissue model screenings using different culture environments. The technology can be parallelized for automated read-out of different multi-well-plate formats.
我们展示了一种新型光学设备，该设备基于散斑图案变化开发，用于监测各种培养环境中扩展3D组织模型的动态行为。所呈现的结果指出了该技术在检测、准确性、灵敏度、合理的读出速度以及心脏收缩测量和监测的可重复性方面的优势。我们表明，光学读出技术适用于长时间监测和药物筛选。本文对该方法进行了讨论，并与其他技术，特别是钙成像技术进行了比较。该设备灵活，易于适应使用不同培养环境的2D和3D组织模型筛选。该技术可以并行化，用于自动读出不同的多孔板格式。
