Mishra Megha, Verma Rakesh Kumar, Gaur R K
1Department of Biosciences, School of Sciences, Mody University of Science and Technology, Lakshmangarh, Sikar, Rajasthan 332311 India.
2Department of Biotechnology, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh 273009 India.
3 Biotech. 2020 Apr;10(4):169. doi: 10.1007/s13205-020-2159-9. Epub 2020 Mar 16.
A monopartite begomovirus associated with betasatellite was identified from (Cape Daisy) showing severe yellowing vein net symptoms in Rajasthan, India through molecular characterization. The DNA-A shared the highest nucleotide (96.61%) identity to (KM880103), while the betasatellite depicted the highest sequence similarity (99.28%) to (JF706231, 99.28%). Based on the sequence identity with other begomoviruses known to date, they were recognized as (CDI, MH355641) and (CDB1, MH355642), respectively. Phylogenetic analysis showed that DNA-A (CD1) clustered with ChiLCV Goa (KP235539), whereas the betasatellite (CDB1) clustered with ChiLCB Jodhapur (JF70623). Recombination events were observed among the clades of ChiLCV, showing intragenic recombination in Rep (C1) and coat protein (V1/AV1) regions. To our knowledge, this is the first report of ChiLC begomovirus strain affecting .
通过分子鉴定，从印度拉贾斯坦邦表现出严重黄化叶脉网症状的（海角雏菊）中鉴定出一种与β卫星相关的单分体双生病毒。DNA-A与（KM880103）的核苷酸同一性最高（96.61%），而β卫星与（JF706231，99.28%）的序列相似性最高。根据与迄今已知的其他双生病毒的序列同一性，它们分别被鉴定为（CDI，MH355641）和（CDB1，MH355642）。系统发育分析表明，DNA-A（CD1）与奇尔卡湖病毒果阿株（KP235539）聚类，而β卫星（CDB1）与焦特布尔奇尔卡湖病毒β卫星（JF70623）聚类。在奇尔卡湖病毒的进化枝中观察到重组事件，在复制酶（C1）和外壳蛋白（V1/AV1）区域显示基因内重组。据我们所知，这是关于影响（海角雏菊）的奇尔卡湖双生病毒株的首次报道。
