Jayanthi P, Geetanjali A Swapna
Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, 603 203, Tamil Nadu, India.
Int Microbiol. 2025 Apr;28(4):863-876. doi: 10.1007/s10123-024-00580-0. Epub 2024 Sep 4.
Chilli leaf curl disease (ChiLCD), which is a significant problem in chilli cultivation, is caused by begomoviruses that are transmitted by the whitefly Bemisia tabaci. This disease leads to severe impacts on crop yields. To determine the incidence of begomovirus in the chilli crop, infected chilli leaf samples exhibiting symptoms such as curling, yellowing, reduced leaf size, and overall stunted growth were collected from various districts of Tamil Nadu, namely, Coimbatore, Dharmapuri, Kancheepuram, Karur, Salem, Krishnagiri, Thoothukudi, Thiruvallur, Tiruchirappalli, Virudhunagar, Tiruvannamalai, Tenkasi, and Vellore, during the years 2018-2022. To determine the complete genome sequence of the begomoviruses, the rolling circle amplification (RCA) method was used to clone and sequence the begomovirus genomes from the chilli samples collected from various districts of Tamil Nadu. Here we characterized 17 DNA A genome sequences and 12 betasatellite sequences. BLAST results of the DNA A genome sequences revealed nucleotide identities ranging from 94.2 to 99.7% with five distinct begomovirus species of chilli, namely, chilli leaf curl Salem virus (HM007119), chilli leaf curl virus Bhavanisagar (NC_055130), chilli leaf curl Ahmedabad virus (MW795666), chilli leaf curl virus (NC_055131), and chilli leaf curl Sri Lanka virus (JN555600). BLAST results of the betasatellite sequences showed nucleotide identities of 96 to 98.8% with the tomato leaf curl Bangladesh betasatellite (MZ151286). In the present study, five distinct begomovirus species and one associated betasatellite were found to infect chilli crops in Tamil Nadu. This finding indicates a changing pattern of begomovirus occurrence in the different districts of Tamil Nadu. This study highlights the prevalence of chilli-infecting begomoviruses in the major chilli growing districts of Tamil Nadu, the identification of begomovirus species, and the significance of understanding and managing these viruses to safeguard chilli cultivation in the region.
辣椒卷叶病（ChiLCD）是辣椒种植中的一个重大问题，由烟粉虱传播的双生病毒引起。这种病害对作物产量造成严重影响。为了确定辣椒作物中双生病毒的发生率，在2018年至2022年期间，从泰米尔纳德邦的各个地区，即哥印拜陀、达摩布里、金奈、卡鲁尔、塞勒姆、克里希纳吉里、杜蒂戈林、蒂鲁瓦勒尔、蒂鲁吉拉伯利、维鲁德讷加尔、蒂鲁万纳马莱、坦卡西和韦洛尔，采集了表现出卷曲、发黄、叶片尺寸减小和整体生长发育不良等症状的受感染辣椒叶片样本。为了确定双生病毒的完整基因组序列，采用滚环扩增（RCA）方法对从泰米尔纳德邦各个地区采集的辣椒样本中的双生病毒基因组进行克隆和测序。在此，我们对17个DNA A基因组序列和12个卫星DNA序列进行了特征分析。DNA A基因组序列的BLAST结果显示，与五种不同的辣椒双生病毒，即辣椒卷叶塞勒姆病毒（HM007119）、辣椒卷叶病毒巴瓦尼萨加尔（NC_055130）、辣椒卷叶艾哈迈达巴德病毒（MW795666）、辣椒卷叶病毒（NC_055131）和辣椒卷叶斯里兰卡病毒（JN555600）的核苷酸同一性在94.2%至99.7%之间。卫星DNA序列的BLAST结果显示，与番茄卷叶孟加拉卫星DNA（MZ151286）的核苷酸同一性为96%至98.8%。在本研究中，发现五种不同的双生病毒和一种相关卫星DNA感染了泰米尔纳德邦的辣椒作物。这一发现表明泰米尔纳德邦不同地区双生病毒的发生模式正在发生变化。本研究强调了泰米尔纳德邦主要辣椒种植区中感染辣椒的双生病毒的流行情况、双生病毒种类的鉴定，以及了解和管理这些病毒对保护该地区辣椒种植的重要性。
