Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, China.
Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
J Anim Physiol Anim Nutr (Berl). 2020 Sep;104(5):1410-1423. doi: 10.1111/jpn.13326. Epub 2020 Mar 24.
The addition of probiotics in swine nutrition is known to positively influence both health and growth. The current study investigates differences in the hepatic transcriptome profiles between weaned piglets supplemented with Clostridium butyricum (C. butyricum) and control animals that received no probiotic. The liver is an important metabolic organ that plays a critical role in oxidizing triglycerides for energy production, lipid synthesis and degradation, as well as immune regulation in animals. RNA-Seq analysis was carried out on total RNA harvested from the liver of piglets fed with (n = 3) or without (n = 3) 5 × 10 C. butyricum CFU/g. Compared to the control piglets, 588 of the genes examined (352 up-regulated and 236 down-regulated) were significantly differentially expressed at a fold change > 2 and p < .05 in animals fed with C. butyricum. Quantitative real-time reverse transcription PCR (qRT-PCR) analysis was further used to validate the microarray expression results for 28 genes tested. The functional annotation analyses revealed several genes, processes and pathways with putative involvement in piglet growth and performance. Feeding swine with 5 × 10 C. butyricum CFU/g appears to reinforce their immune status as well as foster the cell cycle and improve the metabolism of carbohydrates, lipids and amino acids. This study provides valuable information about the expression profiles of mRNAs in piglet liver and in-depth functional investigations of these mRNAs that could provide new insights into the molecular networks of growth, immune responses and nutrient metabolism in the porcine liver.
在猪的营养中添加益生菌已知可以积极影响健康和生长。本研究调查了补充丁酸梭菌（C. butyricum）的断奶仔猪与未接受益生菌的对照组动物之间肝脏转录组谱的差异。肝脏是一个重要的代谢器官，在动物中起着氧化甘油三酯产生能量、脂质合成和降解以及免疫调节的关键作用。对用（n = 3）或不用（n = 3）5×10 CFU/g C. butyricum 喂养的仔猪肝脏中总 RNA 进行了 RNA-Seq 分析。与对照组仔猪相比，用 C. butyricum 喂养的仔猪中有 588 个基因（352 个上调和 236 个下调）的表达显著差异，fold change>2，p<0.05。定量实时逆转录 PCR（qRT-PCR）分析进一步用于验证微阵列表达结果在 28 个测试基因。功能注释分析揭示了几个可能涉及仔猪生长和性能的基因、过程和途径。用 5×10 CFU/g C. butyricum 喂养猪似乎增强了它们的免疫状态，促进了细胞周期，并改善了碳水化合物、脂质和氨基酸的代谢。本研究提供了关于仔猪肝脏中 mRNA 表达谱的有价值信息，并对这些 mRNA 进行了深入的功能研究，为猪肝脏中的生长、免疫反应和营养代谢的分子网络提供了新的见解。
