Cao Linyuan, Liu Zhemin, Yu Ying, Liang Qingping, Wei Xinyi, Sun Han, Fang Yangtao, Zhu Changliang, Kong Qing, Fu Xiaodan, Mou Haijin
College of Food Science and Engineering, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, Shandong, People's Republic of China.
State Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, Jiangxi, People's Republic of China.
Appl Microbiol Biotechnol. 2023 Mar;107(5-6):1903-1916. doi: 10.1007/s00253-023-12436-0. Epub 2023 Feb 16.
Diarrhea is a global problem that causes economic losses in the pig industry. There is a growing attention on finding new alternatives to antibiotics to solve this problem. Hence, this study aimed to compare the prebiotic activity of low-molecular-weight hydrolyzed guar gum (GMPS) with commercial manno-oligosaccharide (MOS) and galacto-oligosaccharide (GOS). We further identified their combined effects along with probiotic Clostridium butyricum on regulating the intestinal microbiota of diarrheal piglet by in vitro fermentation. All the tested non-digestible carbohydrates (NDCs) showed favorable short-chain fatty acid-producing activity, and GOS and GMPS showed the highest production of lactate and butyrate, respectively. After 48 h of fermentation, the greatest enhancement in the abundance of Clostridium sensu stricto 1 was observed with the combination of GMPS and C. butyricum. Notably, all the selected NDCs significantly decreased the abundances of pathogenic bacteria genera Escherichia-Shigella and Fusobacterium and reduced the production of potentially toxic metabolites, including ammonia nitrogen, indole, and skatole. These findings demonstrated that by associating with the chemical structure, GMPS exhibited butyrogenic effects in stimulating the proliferation of C. butyricum. Thus, our results provided a theoretical foundation for further application of galactosyl and mannosyl NDCs in the livestock industry. KEY POINTS: • Galactosyl and mannosyl NDCs showed selective prebiotic effects. • GMPS, GOS, and MOS reduced pathogenic bacteria and toxic metabolites production. • GMPS specifically enhanced the Clostridium sensu stricto 1 and butyrate production.
腹泻是一个全球性问题，给养猪业造成经济损失。人们越来越关注寻找抗生素的新替代品来解决这一问题。因此，本研究旨在比较低分子量水解瓜尔胶（GMPS）与商业甘露寡糖（MOS）和低聚半乳糖（GOS）的益生元活性。我们进一步通过体外发酵确定了它们与益生菌丁酸梭菌联合对腹泻仔猪肠道微生物群的调节作用。所有测试的不可消化碳水化合物（NDCs）均表现出良好的短链脂肪酸产生活性，GOS和GMPS分别表现出最高的乳酸和丁酸产量。发酵48小时后，GMPS与丁酸梭菌联合使用时，观察到严格意义上的梭菌属1丰度增加最为显著。值得注意的是，所有选定的NDCs均显著降低了病原菌属大肠杆菌-志贺氏菌属和梭杆菌属的丰度，并减少了包括氨氮、吲哚和粪臭素在内的潜在有毒代谢物的产生。这些发现表明，GMPS通过与化学结构相关联，在刺激丁酸梭菌增殖方面表现出产丁酸作用。因此，我们的结果为半乳糖基和甘露糖基NDCs在畜牧业中的进一步应用提供了理论基础。要点：•半乳糖基和甘露糖基NDCs表现出选择性益生元作用。•GMPS、GOS和MOS减少了病原菌和有毒代谢物的产生。•GMPS特异性增强了严格意义上的梭菌属1和丁酸的产生。
