Selivanova Natalia M, Gubaidullin Aidar T, Galyametdinov Yuriy G
Kazan National Research Technological University, 68 Karl Marx Street, 420015 Kazan, Russia.
Arbuzov Institute of Organic and Physical Chemistry of the Kazan Scientific Center of the Russian Academy of Sciences, 8 Akademika Arbuzov Street, 420088 Kazan, Russia.
J Phys Chem B. 2020 Apr 2;124(13):2715-2722. doi: 10.1021/acs.jpcb.0c00512. Epub 2020 Mar 24.
An approach to incorporate a bioactive hydrophobic substance, CHNO tetrapeptide (TP), into the structure of the hexagonal mesophases CEO/HO and CEO/La(III)/HO was proposed. Concentration and temperature ranges of mesophases in the CEO/HO/TP and CEO/La(III)/HO/TP systems were established. The analysis of the X-ray diffraction data revealed a change in the structural characteristics of mesophases in the presence of tetrapeptide. Formation of a denser packing of molecules in the mesophases with TP was detected. Based on the FTIR spectroscopy data, intermolecular changes in the systems were examined. Pulsed-gradient spin-echo NMR self-diffusion experiments were performed to characterize the structure of lyomesophases depending on system composition and temperature. The degree of hydration of water molecules in lyomesophases was analyzed. The data confirmed successful incorporation of tetrapeptide into the structure of lyomesophase and, therefore, the possibility of using hexagonal mesophases for both incapsulation and delivery of biomolecules.
提出了一种将生物活性疏水物质CHNO四肽（TP）纳入六方中间相CEO/HO和CEO/La(III)/HO结构的方法。确定了CEO/HO/TP和CEO/La(III)/HO/TP体系中间相的浓度和温度范围。对X射线衍射数据的分析揭示了在四肽存在下中间相结构特征的变化。检测到在含有TP的中间相中分子形成了更致密的堆积。基于傅里叶变换红外光谱数据，研究了体系中的分子间变化。进行了脉冲梯度自旋回波核磁共振自扩散实验，以表征取决于体系组成和温度的溶致中间相的结构。分析了溶致中间相中水分子的水合程度。数据证实四肽成功地纳入了溶致中间相的结构，因此，六方中间相用于生物分子的包封和递送是可能的。
