Occupational Biochemistry, Regional occupational Health Centre (Southern), Bangalore, Karnataka, India.
Food Protectants and Infestation Control, CSIR - Central Food Technological Research Institute, Mysore, Karnataka, India.
Hum Exp Toxicol. 2020 Sep;39(9):1190-1199. doi: 10.1177/0960327120913080. Epub 2020 Mar 24.
Earlier, we demonstrated that chronic exposure to monocrotophos (MCP) elicits insulin resistance in rats along with increased white adipose tissue (WAT) weights. This study was carried out to delineate the biochemical and molecular changes in adipose tissues of rats subjected to chronic exposure to MCP (0.9 and 1.8 mg/kg bw/d for 180 days). Pesticide-treated rats exhibited increased fasting glucose and hyperinsulinemia as well as dyslipidemia. Tumor necrosis factor-alpha and leptin levels were elevated, while adiponectin level was suppressed in plasma of treated rats. MCP treatment caused discernable increase in the weights of perirenal and epididymal WAT. Acetyl coenzyme A carboxylase, fatty acid synthase, glyceraldehyde-3-phosphate dehydrogenase, lipin-1, and lipolytic activities were elevated in the WAT of MCP-treated rats. Corroborative changes were observed in the expression profile of proteins that are involved in lipogenesis and adipose tissue differentiation. Our results clearly demonstrate that long-term exposure to organophosphorus insecticides (OPIs) such as MCP has far-reaching consequences on metabolic health as evidenced by the association of adipogenic outcomes with insulin resistance, hyperinsulinemia, endocrine dysregulations, and dyslipidemia. Taken together, our results suggest that long-term exposure to OPI may be a risk factor for metabolic dysregulations.
先前，我们证实了长期接触久效磷（MCP）会导致大鼠产生胰岛素抵抗，同时增加白色脂肪组织（WAT）的重量。本研究旨在描述慢性接触 MCP（0.9 和 1.8 mg/kg bw/d，持续 180 天）的大鼠脂肪组织中的生化和分子变化。经农药处理的大鼠表现出空腹血糖升高和高胰岛素血症以及血脂异常。肿瘤坏死因子-α和瘦素水平升高，而血浆中的脂联素水平降低。MCP 处理导致大鼠肾周和附睾 WAT 的重量明显增加。乙酰辅酶 A 羧化酶、脂肪酸合酶、甘油醛-3-磷酸脱氢酶、脂联素-1 和脂肪分解活性在 MCP 处理的大鼠 WAT 中升高。参与脂肪生成和脂肪组织分化的蛋白质的表达谱也观察到了协同变化。我们的结果清楚地表明，长期接触有机磷杀虫剂（OPI），如 MCP，对代谢健康有深远的影响，这与脂肪生成结果与胰岛素抵抗、高胰岛素血症、内分泌失调和血脂异常有关。总之，我们的结果表明，长期接触 OPI 可能是代谢失调的一个危险因素。
