超高效液相色谱-串联质谱法测定 Minas Frescal 奶酪中的聚醚离子载体残留。

Polyether ionophores residues in Minas Frescal cheese by UHPLC-MS/MS.

机构信息

Department of Food Science, School of Food Engineering, University of Campinas - UNICAMP , Campinas, Brazil.

出版信息

Food Addit Contam Part B Surveill. 2020 Jun;13(2):130-138. doi: 10.1080/19393210.2020.1739149. Epub 2020 Mar 24.

DOI:10.1080/19393210.2020.1739149

PMID:32207380

Abstract

An analytical method was developed and validated for the determination of three polyether ionophores (monensin, lasalocid, and salinomycin) in 60 samples of Brazilian Minas Frescal cheese by UHPLC-MS/MS. Linearity ranged from 1 to 8 μg kg for monensin and salinomycin, and from 0.50 to 4 μg kg for lasalocid. Limits of detection and quantitation were 0.50 μg kg and 1 μg kg, respectively, for both monensin and salinomycin, and 0.25 μg kg and 0.50 μg kg, respectively, for lasalocid. Recoveries were between 69% and 84% with coefficients of variation up to 16.28% for repeatability and 13.79% for intermediate precision. A total of 60 samples of Minas Frescal cheese were analysed and only monensin residues were found. Monensin was detected in 55% of the samples and quantified in 5 of them at mean levels varying from 1.00 to 1.73 μg kg. The proposed method demonstrated the suitability for monitoring these substances in cheese.

摘要

建立并验证了一种超高效液相色谱-串联质谱法，用于测定 60 份巴西 Minas Frescal 奶酪中三种聚醚离子载体（莫能菌素、拉沙里菌素和盐霉素）的含量。莫能菌素和盐霉素的线性范围为 1 至 8μg kg，拉沙里菌素的线性范围为 0.50 至 4μg kg。莫能菌素和盐霉素的检测限和定量限均为 0.50μg kg，拉沙里菌素的检测限和定量限分别为 0.25μg kg 和 0.50μg kg。回收率在 69%至 84%之间，重复性的变异系数高达 16.28%，中间精密度的变异系数高达 13.79%。分析了 60 份 Minas Frescal 奶酪样品，仅发现莫能菌素残留。55%的样品中检测到莫能菌素，其中 5 份样品的含量在 1.00 至 1.73μg kg 之间。该方法适用于奶酪中这些物质的监测。