流式细胞术分析筛查宫颈癌患者宫颈固有细胞表面多能跨膜糖蛋白标志物

Flow Cytometry Characterization of Pluripotent Transmembrane Glycoproteins on Resident Cervix Uteri Cells in Patients Screened for Cervical Cancer.

机构信息

Obstetrics and Gynecology, Department of Woman, Child and General and Specialized Surgery, University of Campania "Luigi Vanvitelli", Naples, Italy.

Department of Advanced Medical and Surgical Sciences. U.O.C. Division of Clinical Immunology, Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Regional Reference Laboratory of Transplant Immunology (LIT), University of Campania "Luigi Vanvitelli", Naples, Italy.

出版信息

Cancer Invest. 2020 Apr;38(4):228-239. doi: 10.1080/07357907.2020.1742349. Epub 2020 Apr 13.

DOI:10.1080/07357907.2020.1742349

PMID:32208057

Abstract

The aim of this study was to characterize both by flow cytometry analysis and immunohistochemistry cervix uteri cells of women screened for cervical intraepithelial neoplasia (CIN) in comparison to a group without CIN by using mesenchymal stem cell-like and hematopoietic lineage markers. A significant expression for CD29, CD38, HLA-I, and HLA-II was correlated positively to the CIN degree and it was more relevant in patients positive for human papilloma virus (HPV). Thus, identification and detailed characterization of pluripotent resident in cells could be a promising therapeutic target.

摘要

本研究旨在通过流式细胞术分析和免疫组织化学方法，使用间充质干细胞样和造血谱系标记物，对因宫颈上皮内瘤变（CIN）而接受筛查的女性的宫颈细胞进行特征描述，并与无 CIN 的女性进行比较。CD29、CD38、HLA-I 和 HLA-II 的显著表达与 CIN 程度呈正相关，在 HPV 阳性患者中更为显著。因此，鉴定和详细描述多能常驻细胞可能是一个有前途的治疗靶点。