Faculty of Medicine, Department of Obstetrics and Gynecology, University of Tokyo, Hongo, Bunkyo-ku, Japan.
Am J Reprod Immunol. 2011 Nov;66(5):435-43. doi: 10.1111/j.1600-0897.2011.01041.x. Epub 2011 Jul 12.
Mucosal T cells are the most likely direct effectors in host anti-human papillomavirus adaptive immunity and regression of cervical intraepithelial neoplasia (CIN) lesions. There are no studies addressing intraepithelial lymphocytes (IELs) in CIN lesions.
Cervical lymphocytes were collected using cytobrushes from patients with CIN and analyzed by FACS analysis. Comparisons were made between populations of cervical T cells in CIN regressors and non-regressors.
A median of 74% of cervical lymphocytes were CD3(+) T cells. Populations of integrin αEβ7(+) IEL in CIN lesions varied markedly among patients (6-57%). Approximately half of integrin β7(+) T cells were CD45RA-negative memory T cells. The number of integrin αEβ7(+) cells among cervical T cells was significantly higher in CIN regressors when compared to non-regressors.
Higher cervical IEL numbers are associated with spontaneous regression of CIN. Accumulation of cervical integrin αEβ7(+) IEL may be necessary for local adaptive effector functions.
黏膜 T 细胞是宿主抗人乳头瘤病毒适应性免疫以及宫颈上皮内瘤变（CIN）消退的最直接效应细胞。目前还没有研究涉及 CIN 病变中的上皮内淋巴细胞（IEL）。
使用细胞刷从 CIN 患者中收集宫颈淋巴细胞，并通过 FACS 分析进行分析。比较 CIN 消退者和非消退者宫颈 T 细胞群体。
中位数为 74%的宫颈淋巴细胞为 CD3(+) T 细胞。CIN 病变中整合素 αEβ7(+) IEL 的群体在患者之间差异很大（6-57%）。大约一半的整合素 β7(+) T 细胞为 CD45RA 阴性记忆 T 细胞。与非消退者相比，CIN 消退者宫颈 T 细胞中的整合素 αEβ7(+)细胞数量明显更高。
更高的宫颈 IEL 数量与 CIN 的自发消退有关。宫颈整合素 αEβ7(+) IEL 的积累可能是局部适应性效应功能所必需的。
