Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita, La Paz, B.C.S. 23096, Mexico.
Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita, La Paz, B.C.S. 23096, Mexico; Universidad Autónoma de Baja California Sur, Depto. de Biología Marina, Posgrado en Ciencias Marinas y Costeras (CIMACO), Carretera al Sur km 5.5, C.P 23080, La Paz, Baja California Sur, Mexico.
Fish Shellfish Immunol. 2020 May;100:418-426. doi: 10.1016/j.fsi.2020.03.045. Epub 2020 Mar 21.
In Mexican herbal medicines or natural remedies, Turnera diffusa (Turneraceae) known as "Damiana de California", has ethnopharmacological relevance, including aphrodisiac, diuretic, and antimicrobial activities. To explore the immunological effect of infusion and methanolic extracts from Damiana de California, this study investigated its chemical, biological, antimicrobial and immunological properties in Longfin yellowtail Seriola rivoliana leukocytes. The analysis of chemical compounds revealed a considerable level of total phenolic and flavonoid contents in the infusion compared with methanolic extract. Furthermore, the antioxidant activity showed high hydroxyl radical scavenging activity in infusion extract compared with BHT positive control. Superoxide radical scavenging activity and ion chelation were higher in methanolic extract followed by infusion treatment. Interestingly, notable antimicrobial activity was observed in both extracts of T. diffusa against Vibrio parahaemolyticus. An in vitro study was performed using leukocytes of S. rivoliana treated with infusion or methanolic extracts at 12.5, 25 and 50 μg/mL for 24 h. Remarkably, infusion extract induced proliferation at any concentration but not the methanolic extract, which was diminished in a dose-dependent fashion. The immunostimulation study demonstrated that the phagocytosis activity increased in those leukocytes stimulated with methanolic extract but diminished the respiratory burst activity, in contrast to the activity observed in those leukocytes stimulated with infusion treatment. Finally, leukocytes incubated with the extracts and confronted with V.parahaemolyticus up-regulated the transcription of proinflammatory cytokine IL-1β gene in a dose response relationship. These findings suggest that the infusion treatment has potential therapeutic properties, promoting the antioxidant capacity and enhancing immune parameters in Longfin yellowtail S. rivoliana.
在墨西哥草药或天然药物中，Turnera diffusa（Turneraceae）被称为“加利福尼亚 Damiana”，具有民族药理学相关性，包括催情、利尿和抗菌活性。为了探索加利福尼亚 Damiana 的浸液和甲醇提取物的免疫作用，本研究调查了其在长鳍黄尾 Seriola rivoliana 白细胞中的化学、生物学、抗菌和免疫特性。化合物分析显示，与甲醇提取物相比，浸液中的总酚和类黄酮含量相当高。此外，与 BHT 阳性对照相比，浸液提取物具有较高的羟基自由基清除活性，显示出较高的抗氧化活性。超氧自由基清除活性和离子螯合在甲醇提取物中较高，其次是浸液处理。有趣的是，两种 T. diffusa 提取物对副溶血弧菌均表现出显著的抗菌活性。在体外研究中，使用 S. rivoliana 的白细胞，用 12.5、25 和 50μg/mL 的浸液或甲醇提取物处理 24 小时。值得注意的是，浸液提取物在任何浓度下都能诱导增殖，但甲醇提取物则不能，且呈剂量依赖性减少。免疫刺激研究表明，与甲醇提取物刺激的白细胞相比，浸液处理刺激的白细胞的吞噬活性增加，但呼吸爆发活性降低。最后，与提取物孵育并与 V.parahaemolyticus 对抗的白细胞上调促炎细胞因子 IL-1β 基因的转录，呈剂量反应关系。这些发现表明，浸液处理具有潜在的治疗特性，可促进抗氧化能力，并增强长鳍黄尾 S. rivoliana 的免疫参数。
