Kritzer M F, Innis R B, Goldman-Rakic P S
Section of Neuroanatomy, Yale University School of Medicine, New Haven, Connecticut 06510.
J Comp Neurol. 1988 Oct 8;276(2):219-30. doi: 10.1002/cne.902760206.
Cholecystokinin (CCK) binding sites were localized in the hippocampus, amygdala, and medial temporal cortices of macaque monkeys by using techniques of in vitro receptor autoradiography. Binding sites were labeled with 3H-CCK-8 and 125I-CCK-33, and nonspecific binding was assessed in the presence of 1 microM CCK-8. Comparison of autoradiograms with Nissl-stained sections allowed precise correlation of autoradiographic grain distribution with cytoarchitecture. CCK binding in the amygdala varied among nuclear subdivisions. It was dense in the lateral, basomedial, endopiriform, and cortical nuclei, in the parvicellular portion of the accessory basal nucleus, the periamygdaloid cortex, the cortical transition area, and in the amygdalohippocampal area. Labeling was sparse in the central, medial, and basolateral nuclei as well as in the magnocellular accessory basal nucleus. In the hippocampal formation, a single dense band of CCK binding was observed over the granule cell layer and adjacent few millimeters of the molecular layer of the dentate gyrus, while in the polymorph and remaining portions of this layer binding was of very low density. Prominent label over the pyramidal layer in the presubiculum clearly distinguished this region from the adjacent subiculum in which binding just exceeded background levels. Moderate to light label was observed in the hilus and stratum pyramidale of CA3, CA2, and CA1, while other hippocampal layers showed minimal specific binding. Variation in CCK binding in the medial temporal cortex showed close correspondence to cytoarchitectonic subdivisions. In entorhinal cortex, for example, binding was concentrated in layers III-VI while label in area 35 was prominent in all laminae except layer IV. Area TH of von Bonin and Bailey ('47) was distinguished from other regions by evenly distributed binding across all layers, while in area TF a bilaminar pattern of label in layers II and IV was observed. The highly specific patterns of CCK binding in amygdala and transitional cortices of the medial temporal lobe can be related to terminal fields of neo- and allocortical afferents to these regions, while label in the hippocampal formation coincides with the terminals of intrinsic neurons which ramify among the somata of cells that are targets of neocortical afferents. Thus, in all structures of the medial temporal lobe the disposition of peptidergic binding sites suggests that CCKergic systems may be important in the modulation of cortical afferents.
采用体外受体放射自显影技术，在猕猴的海马、杏仁核及颞叶内侧皮质中定位了胆囊收缩素（CCK）结合位点。用³H-CCK-8和¹²⁵I-CCK-33标记结合位点，并在1微摩尔CCK-8存在的情况下评估非特异性结合。将放射自显影片与尼氏染色切片进行比较，可使放射自显影颗粒分布与细胞结构精确对应。杏仁核中CCK结合在核亚群之间存在差异。在外侧核、基底内侧核、梨状内核、皮质核、副基底核的小细胞部分、杏仁周皮质、皮质过渡区以及杏仁海马区中结合密集。在中央核、内侧核、基底外侧核以及大细胞副基底核中标记稀疏。在海马结构中，在齿状回颗粒细胞层及相邻几毫米的分子层上观察到一条单一的CCK结合密集带，而在该层的多形层及其余部分结合密度非常低。前下托锥体细胞层上的明显标记清楚地将该区域与相邻的下托区分开来，下托中的结合仅略高于背景水平。在CA3、CA2和CA1的海马回及锥体层中观察到中度至轻度标记，而其他海马层显示出最小的特异性结合。颞叶内侧皮质中CCK结合的变化与细胞结构亚区密切对应。例如，在内嗅皮质中，结合集中在III - VI层，而在35区，除IV层外所有层中的标记都很突出。冯·博宁和贝利（1947年）的TH区与其他区域的区别在于所有层中结合均匀分布，而在TF区，在II层和IV层观察到双层标记模式。杏仁核和颞叶内侧过渡皮质中CCK结合的高度特异性模式可能与新皮质和旧皮质传入这些区域的终末场有关，而海马结构中的标记与内在神经元的终末一致，这些内在神经元在新皮质传入靶细胞的胞体之间分支。因此，在颞叶内侧的所有结构中，肽能结合位点的分布表明CCK能系统可能在皮质传入的调节中起重要作用。
