Vollmer G, Wünsche W O, Knuppen R
Institut für Biochemische Endokrinologie, Medizinische Universität zu Lübeck.
J Clin Chem Clin Biochem. 1988 Jul;26(7):453-8. doi: 10.1515/cclm.1988.26.7.453.
The activity of the oestradiol-17 beta hydroxysteroid dehydrogenase in human endometrial and breast cancer specimens was determined by the NAD-dependent conversion of oestradiol-17 beta to oestrone. The sensitivity of the determination was improved by the separation of the hormones by HPLC. We are now able to determine oestradiol-17 beta hydroxysteroid dehydrogenase quantitatively in cryostat sections. A clear correlation of serum progesterone levels and oestradiol-17 beta hydroxysteroid dehydrogenase activity in the endometrium was demonstrated, and we found a more than 30-fold increase in enzyme activity after the progesterone surge. In contrast, in breast cancer samples, we found no correlation between oestradiol-17 beta hydroxysteroid dehydrogenase and the measured serum parameters.
通过NAD依赖的雌二醇-17β向雌酮的转化，测定人子宫内膜和乳腺癌标本中雌二醇-17β羟类固醇脱氢酶的活性。通过高效液相色谱法分离激素提高了测定的灵敏度。我们现在能够在冷冻切片中定量测定雌二醇-17β羟类固醇脱氢酶。结果表明，血清孕酮水平与子宫内膜中雌二醇-17β羟类固醇脱氢酶活性存在明显相关性，并且我们发现孕酮激增后酶活性增加了30多倍。相比之下，在乳腺癌样本中，我们发现雌二醇-17β羟类固醇脱氢酶与所测血清参数之间没有相关性。
