Neuroscience Department, International School for Advanced Studies (SISSA), via Bonomea 265, 34136, Trieste, TS, Italy.
SPINAL (Spinal Person Injury Neurorehabilitation Applied Laboratory), Istituto di Medicina Fisica e Riabilitazione (IMFR), via Gervasutta 48, Udine, UD, Italy.
Cell Mol Neurobiol. 2021 Jan;41(1):185-190. doi: 10.1007/s10571-020-00831-4. Epub 2020 Mar 24.
Motoneuron activity is modulated by histamine receptors. While H and H receptors have been widely explored, H histamine receptors (HRs) have not been sufficiently characterized. This paper targets the effects of the selective activation of HRs and their expression on the membranes of large ventral horn cells. The application of selective pharmacological agents to spinal cords isolated from neonatal rats was used to identify the presence of functional HRs on the membrane of physiologically identified lumbar motoneurons. Intra and extracellular recordings revealed that HR agonist, α-methylhistamine, depolarized both single motoneurons and ventral roots, even in the presence of tetrodotoxin, an effect prevented by HR antagonist, thioperamide. Finally, immunohistochemistry located the expression of HRs on a subpopulation of large cells in lamina IX. This study identifies HRs as a new exploitable pharmacological target against motor disturbances.
运动神经元的活动受组胺受体的调节。虽然 H 1 和 H 2 受体已被广泛研究，但 H 3 组胺受体 (HRs) 尚未得到充分表征。本文针对 HRs 的选择性激活及其在大型腹角细胞膜上的表达的影响进行了研究。应用选择性药理学制剂对从新生大鼠分离的脊髓进行处理，以鉴定生理鉴定的腰运动神经元膜上是否存在功能性 HRs。细胞内和细胞外记录显示，HR 激动剂 α-甲基组胺可使单个运动神经元和腹根去极化，即使在存在河豚毒素的情况下也是如此，HR 拮抗剂噻庚啶可防止这种作用。最后，免疫组织化学将 HRs 的表达定位在层 IX 的一个大细胞亚群上。这项研究确定 HRs 是一种针对运动障碍的新的可开发药理学靶点。
