Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich, 8093, Zurich, Switzerland.
Angew Chem Int Ed Engl. 2020 May 18;59(21):8138-8142. doi: 10.1002/anie.202000835. Epub 2020 Apr 15.
The assembly of protein and inorganic nanoparticles represents an attractive approach to generate composite materials with multiple functions. Herein, we functionalize inorganic nanoparticles with intrinsically disordered protein domains associated with the formation of membraneless compartments in cells. These protein sequences, defined as low complexity domains (LCDs), encode intermolecular interactions that drive highly controlled, dynamic self-assembly in response to environmental changes. We show that the properties of the LCDs can be transferred to inorganic nanoparticles, inducing controlled phase separation that is dynamic and responsive to ionic strength and pH. Specifically, we hybridize magnetic nanoparticles with multi-domain proteins consisting of LCD domains and a globular enzyme, generating dynamic protein-composite compartments that locally confine hybrid chemoenzymatic reactions and respond to external magnetic fields and changes in solution conditions.
蛋白质和无机纳米粒子的组装代表了一种很有吸引力的方法，可以生成具有多种功能的复合材料。在此，我们用与细胞中无膜隔室形成有关的固有无序蛋白结构域对无机纳米粒子进行功能化。这些蛋白序列被定义为低复杂度结构域（LCD），它们编码的分子间相互作用可以在响应环境变化时驱动高度可控的、动态的自组装。我们表明，LCD 的性质可以转移到无机纳米粒子上，诱导动态且对离子强度和 pH 值有响应的可控相分离。具体来说，我们将磁性纳米粒子与由 LCD 结构域和球状酶组成的多结构域蛋白杂交，生成可以局部限制混合化学酶反应并响应外磁场和溶液条件变化的动态蛋白复合隔室。
