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本文引用的文献

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Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain.由猪水疱病病毒HK/70株全长互补DNA产生的感染性病毒。
Chin Sci Bull. 2006;51(17):2072-2078. doi: 10.1007/s11434-006-2095-z.
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Overexpression of celB gene coding for beta-glucosidase from Pyrococcus furiosus using a baculovirus expression vector system in silkworm, Bombyx mori.在家蚕（Bombyx mori）中使用杆状病毒表达载体系统过表达编码来自嗜热栖热菌（Pyrococcus furiosus）的β-葡萄糖苷酶的celB基因。
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[Construction and identification of recombinant adenovirus containing the polyproteins coding regions of O type foot-and-mouth disease virus by homologous recombination in Escherichia coli].通过大肠杆菌同源重组构建并鉴定含O型口蹄疫病毒多聚蛋白编码区的重组腺病毒
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Rapid protection of cattle from direct challenge with foot-and-mouth disease virus (FMDV) by a single inoculation with an adenovirus-vectored FMDV subunit vaccine.通过单次接种腺病毒载体口蹄疫病毒（FMDV）亚单位疫苗，快速保护牛免受口蹄疫病毒直接攻击。
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Immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus O/China99.用口蹄疫病毒O/China99 DNA疫苗接种的豚鼠的免疫反应
Vaccine. 2005 May 9;23(25):3236-42. doi: 10.1016/j.vaccine.2004.03.074.
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Development of transgenic alfalfa plants containing the foot and mouth disease virus structural polyprotein gene P1 and its utilization as an experimental immunogen.含有口蹄疫病毒结构多聚蛋白基因P1的转基因苜蓿植株的培育及其作为实验性免疫原的应用。
Vaccine. 2005 Mar 7;23(15):1838-43. doi: 10.1016/j.vaccine.2004.11.014.
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Efficient assembly and release of SARS coronavirus-like particles by a heterologous expression system.通过异源表达系统高效组装和释放严重急性呼吸综合征冠状病毒样颗粒。
FEBS Lett. 2004 Oct 8;576(1-2):174-8. doi: 10.1016/j.febslet.2004.09.009.
8
Immediate protection of swine from foot-and-mouth disease: a combination of adenoviruses expressing interferon alpha and a foot-and-mouth disease virus subunit vaccine.猪对口蹄疫的即时保护：表达α干扰素的腺病毒与口蹄疫病毒亚单位疫苗的组合
Vaccine. 2003 Dec 12;22(2):268-79. doi: 10.1016/s0264-410x(03)00560-7.
9
Virus-like particles as immunogens.作为免疫原的病毒样颗粒
Trends Microbiol. 2003 Sep;11(9):438-44. doi: 10.1016/s0966-842x(03)00208-7.
10
Molecular basis of pathogenesis of FMDV.口蹄疫病毒发病机制的分子基础。
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用来自幼虫的口蹄疫病毒重组完整多聚蛋白接种牛后的免疫反应。

Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from larvae.

作者信息

Li ZhiYong, Yi YongZhu, Yin XiangPing, Zhang ZhiFang, Liu JiXing

机构信息

1Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou, 730046 China.

2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.

出版信息

Chin Sci Bull. 2007;52(20):2805-2810. doi: 10.1007/s11434-007-0436-1.

DOI:10.1007/s11434-007-0436-1
PMID:32214727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7089379/
Abstract

The intact open reading frame (ORF) of foot-and-mouth disease virus (FMDV) Asia I/XJ strain was amplified by RT-PCR and inserted into the transfer vector pVL1393 to generate plasmid pVL-ORF. -N cells were transfected with pVL-ORF and linearized -BacPAK6 DNA, and the recombinant silkworm baculovirus -ORF containing the full ORF of FMDV was obtained. The results of indirect immunofluorescence assay (IFA) showed that -ORF could be expressed efficiently in -N cell. After inoculating the early 5th instar larvae of silkworm, the polyprotein of FMDV could be detected by sandwich ELISA and empty capsid-like particles could be observed under the electron microscope. Expression products from silkworm were used as the antigen to immunize the cattle. The specific antibody was induced in all vaccinated animals. The immunized cattle were challenged with the virulent FMDV Asia I/XJ strain, two of the four cattle were completely protected and clinical symptoms were alleviated and delayed in the others. The results suggest that this strategy might be used to develop the new subunit FMDV vaccine.

摘要

通过逆转录聚合酶链反应（RT-PCR）扩增口蹄疫病毒（FMDV）亚洲I型/XJ株的完整开放阅读框（ORF），并将其插入转移载体pVL1393中以构建质粒pVL-ORF。用pVL-ORF和线性化的-BacPAK6 DNA转染-N细胞，获得了含有FMDV完整ORF的重组家蚕杆状病毒-ORF。间接免疫荧光分析（IFA）结果表明，-ORF可在-N细胞中高效表达。接种家蚕5龄初期幼虫后，通过夹心ELISA可检测到FMDV的多聚蛋白，并且在电子显微镜下可观察到空衣壳样颗粒。用家蚕表达产物作为抗原免疫牛。所有接种动物均诱导出特异性抗体。用强毒FMDV亚洲I型/XJ株攻击免疫牛，4头牛中有2头完全得到保护，其他牛的临床症状得到缓解且延迟出现。结果表明，该策略可用于开发新型FMDV亚单位疫苗。