由猪水疱病病毒HK/70株全长互补DNA产生的感染性病毒。
Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain.
作者信息
Zheng Haixue, Liu Xiangtao, Shang Youjun, Wu Jinyan, Bai Xingwen, Jin Ye, Sun Shiqi, Guo Huichen, Tian Hong, Feng Xia, Yin Shuanghui, Guo Jianhong, Cong Guozheng, Liu Zaixin, Chang Huiyun, Ma Junwu, Xie Qingge
机构信息
1Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046 China.
2Graduate School, Chinese Academy of Sciences, Beijing, 100081 China.
出版信息
Chin Sci Bull. 2006;51(17):2072-2078. doi: 10.1007/s11434-006-2095-z.
The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. transcription RNA from pSVOK transfected IBRS-2 cells and the recovered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect immunofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we obtained and the virus derived from pSVOK had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of SVDV.
为研究猪水疱病病毒的抗原性、复制、成熟及致病性，构建了猪水疱病病毒HK/70株的全长cDNA克隆，命名为pSVOK。分离并测序了转染pSVOK的IBRS - 2细胞转录的RNA及回收的病毒RNA，然后进行间接血凝试验、间接免疫荧光试验、电子显微镜检测、50%组织培养感染剂量（TCID）测定和小鼠毒力研究，以研究回收病毒的抗原性和毒力。结果表明，我们获得的感染性克隆及源自pSVOK的病毒与亲代HK/70株具有相同的生物学特性。全长感染性cDNA克隆pSVOK在猪水疱病病毒的抗原性、毒力、发病机制、成熟及复制研究中将非常有用。
Zotero 插件