Zhang Feiyun, Toriyama Shigemitsu, Takahashi Mami
National Institute of Agro-Environmental Sciences, Tsukuba 305-8604, Japan, , , , JP.
J Gen Plant Pathol. 2001;67(1):63-68. doi: 10.1007/PL00012989.
The genome of (RGMoV) comprises 4210 nucleotides. The genomic RNA contains four open reading frames (ORFs). The largest ORF 2 encodes a polyprotein of 947 amino acids (103.6 kDa), which codes for a serine protease and an RNA-dependent RNA polymerase. The viral coat protein is encoded on ORF 4 present at the 3'-proximal region. Other ORFs 1 and 3 encode the predicted 14.6 kDa and 19.8 kDa proteins of unknown function. The consensus signal for frameshifting, heptanucleotide UUUAAAC and a stem-loop structure just downstream is in front of the AUG codon of ORF 3. Analysis of the translation products of RGMoV RNA suggests that the 68 kDa protein may represent a fusion protein of ORF 2-ORF 3 produced by frameshifting. The protease region of the polyprotein and coat protein have a low similarity with that of the sobemoviruses (approximately 25% amino acid identity), while the RNA-dependent RNA polymerase region has particularly strong similarity (54 to 60% of more than 350 amino acid residues). The sequence similarities of RGMoV to the sobemoviruses, together with the characteristic genome organization indicate that RGMoV is a new species of the genus
水稻草状矮化病毒（RGMoV）的基因组由4210个核苷酸组成。基因组RNA包含四个开放阅读框（ORF）。最大的ORF2编码一个由947个氨基酸组成的多聚蛋白（103.6 kDa），其编码一个丝氨酸蛋白酶和一个依赖RNA的RNA聚合酶。病毒外壳蛋白由位于3'-近端区域的ORF4编码。其他的ORF1和ORF3编码预测的功能未知的14.6 kDa和19.8 kDa蛋白。移码的共有信号，七核苷酸UUUAAAC以及紧接下游的一个茎环结构位于ORF3的AUG密码子之前。对RGMoV RNA翻译产物的分析表明，68 kDa蛋白可能代表由移码产生的ORF2-ORF3融合蛋白。多聚蛋白的蛋白酶区域和外壳蛋白与南方菜豆花叶病毒属病毒的相应区域具有较低的相似性（氨基酸同一性约为25%），而依赖RNA的RNA聚合酶区域具有特别高的相似性（超过350个氨基酸残基的54%至60%）。RGMoV与南方菜豆花叶病毒属病毒的序列相似性，连同其特征性的基因组结构表明RGMoV是该属的一个新种
