McGavin W J, Macfarlane S A
Plant Pathology Programme, SCRI, Invergowrie, Dundee DD2 5DA, UK.
Virus Res. 2009 Jan;139(1):10-3. doi: 10.1016/j.virusres.2008.09.004. Epub 2008 Nov 7.
The complete nucleotide sequence of a new member of the unassigned genus Sobemovirus, isolated from raspberry and bramble plants in north east Scotland and given the name Rubus chlorotic mottle virus (RuCMV), was obtained. The virus has a single, positive-strand RNA genome of 3,983 nucleotides and, in common with other sobemoviruses, contains four open reading frames (ORFs) encoding, from 5' to 3', the P1 protein that is likely to be a suppressor of RNA silencing, ORF2a that has homology to serine-proteases, ORF2b that is the probable RNA dependent RNA polymerase, and ORF3 that is the coat protein. ORF2b protein is potentially expressed as a fusion with ORF2a protein by a -1 frameshift at the heptanucleotide sequence UUUAAAC. Phylogenetic analyses showed that RuCMV is a distinct virus not closely related to any of the other sequenced sobemoviruses. Based on the obtained sequence a full-length cDNA copy of RuCMV was cloned and in vitro transcripts derived from this clone were shown to be fully infectious.
从苏格兰东北部的悬钩子和黑莓植物中分离出了未分类的南方菜豆花叶病毒属的一个新成员，其全核苷酸序列已获得，并命名为悬钩子褪绿斑驳病毒（RuCMV）。该病毒具有一个由3983个核苷酸组成的单链正链RNA基因组，与其他南方菜豆花叶病毒一样，包含四个开放阅读框（ORF），从5'到3'依次编码可能是RNA沉默抑制子的P1蛋白、与丝氨酸蛋白酶具有同源性的ORF2a、可能是RNA依赖RNA聚合酶的ORF2b以及外壳蛋白ORF3。ORF2b蛋白可能通过在七核苷酸序列UUUAAAC处发生-1移码而与ORF2a蛋白融合表达。系统发育分析表明，RuCMV是一种独特的病毒，与任何其他已测序的南方菜豆花叶病毒都没有密切关系。基于获得的序列，克隆了RuCMV的全长cDNA拷贝，并且从该克隆获得的体外转录本显示具有完全感染性。
