College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China.
Department of Agricultural and Environmental Engineering, Faculty of Engineering, Bayero University, Kano, Nigeria.
Appl Microbiol Biotechnol. 2020 May;104(10):4505-4513. doi: 10.1007/s00253-020-10393-6. Epub 2020 Mar 25.
This study evaluated the inactivation effect of ultrasonic treatment combined with acidic electrolyzed water (AEW) on Bacillus cereus spores. AEW treatment reduced the spores by 1.05-1.37 log CFU/mL while the sporicidal effect of ultrasound was minor. More strikingly, simultaneous ultrasonic and AEW treatments for 30 min led to 2.29 log CFU/mL reduction and thus, considered a synergistic effect. Flow cytometry combined with SYTO/PI staining analysis revealed that ultrasound hydrolyzed the cortex while the AEW partially damaged the integrity of the inner membrane. Scanning and transmission electron microscopies were used to characterize the ultrastructural changes. The detachment of the exosporium induced by ultrasound was the most apparent difference compared with the control group, and the electron density of spores appeared to be heterogeneous after treatment with AEW. These results indicated that combining ultrasound with AEW is a promising decontamination technology with potential uses in the food industry and environmental remediation.
本研究评估了超声波处理联合酸性电解水（AEW）对蜡样芽孢杆菌孢子的灭活效果。AEW 处理使孢子减少了 1.05-1.37 log CFU/mL，而超声波的杀菌效果较小。更引人注目的是，同时进行 30 分钟的超声和 AEW 处理可导致 2.29 log CFU/mL 的减少，因此被认为具有协同作用。流式细胞术结合 SYTO/PI 染色分析表明，超声波水解了皮层，而 AEW 部分破坏了内膜的完整性。扫描和透射电子显微镜用于表征超微结构变化。与对照组相比，超声诱导的外孢子体脱落是最明显的差异，而 AEW 处理后孢子的电子密度似乎不均匀。这些结果表明，将超声波与 AEW 结合是一种有前途的消毒技术，有望在食品工业和环境修复中得到应用。
