通过分析等电聚焦法对人极低密度脂蛋白中的载脂蛋白C-II和载脂蛋白C-III进行定量分析。
Quantification of apoC-II and apoC-III of human very low density lipoproteins by analytical isoelectric focusing.
作者信息
Catapano A L, Jackson R L, Gilliam E B, Gotto A M, Smith L C
出版信息
J Lipid Res. 1978 Nov;19(8):1047-52.
ApoC-II and apoC-III of human very low density lipoproteins (VLDL) have been quantified by analytical isoelectric focusing (IEF) between pH 4 and 6 in polyacrylamide gels containing 8 M urea. The isoelectric point of apoC-III0 is pH 4.93; apoC-II, pH 4.78; apoC-III1, pH 4.72, and apoC-III2, pH 4.54. ApoC-I is not found in the pH range between pH 4 and 6. Two minor peptides, apoC-IV and apoC-V, with isoelectric points of pH 4.61 and 4.44, respectively, are apoproteins not previously identified. The sensitivity (5--40 microgram) and reproducibility (+/- 8%) of this method allow quantitative analysis of apoC-II and apoC-III distribution in VLDL.
人极低密度脂蛋白（VLDL）中的载脂蛋白C-II（ApoC-II）和载脂蛋白C-III（apoC-III）已通过分析等电聚焦（IEF）在含8M尿素的聚丙烯酰胺凝胶中于pH 4至6之间进行了定量分析。载脂蛋白C-III0的等电点为pH 4.93；载脂蛋白C-II为pH 4.78；载脂蛋白C-III1为pH 4.72，载脂蛋白C-III2为pH 4.54。在pH 4至6的范围内未发现载脂蛋白C-I。两种次要肽段，即等电点分别为pH 4.61和pH 4.44的载脂蛋白C-IV和载脂蛋白C-V，是以前未鉴定出的载脂蛋白。该方法的灵敏度（5 - 40微克）和重现性（±8%）使得能够对极低密度脂蛋白中载脂蛋白C-II和载脂蛋白C-III的分布进行定量分析。
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