Institute of Biophysics, Johannes Kepler University Linz, Gruberstraße 40, Linz 4020, Austria.
A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/5 Leninskiy prospekt, Moscow 119071, Russia.
Phys Rev Lett. 2020 Mar 13;124(10):108102. doi: 10.1103/PhysRevLett.124.108102.
Lipid rafts serve as anchoring platforms for membrane proteins. Thus far they escaped direct observation by light microscopy due to their small size. Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer. Photoswitchable lipids dissolved or reformed the domains. Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains. These sizes are in line with the predicted roles of line tension and membrane undulation as driving forces for alignment.
脂质筏作为膜蛋白的锚定平台。由于它们的体积较小，因此迄今为止，它们一直无法通过光学显微镜进行直接观察。在这里，我们使用不同颜色的染料作为报告分子，用于记录游离双层膜的两个叶片中有序和无序脂质。光可切换脂质溶解或重新形成域。域流动性的测量表明存在 120nm 宽的有序域和 40nm 宽的无序域。这些尺寸与线张力和膜波动作为对齐驱动力的预测作用一致。
