Ai Lili, Wang Hao
Department of Anesthesiology, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
Department of Anesthesiology, the Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
J Int Med Res. 2020 Mar;48(3):300060520904861. doi: 10.1177/0300060520904861.
This study aimed to investigate the effects of propofol and sevoflurane on cytotoxicity of natural killer (NK) cells in patients with gastric cancer.
Patients with gastric cancer were anesthetized by propofol or sevoflurane. Peripheral blood NK cells were isolated and co-cultured with BGC-823 gastric cancer cell culture supernatant, and the rate of apoptosis and effector molecules were analyzed by flow cytometry. Effects of propofol and sevoflurane on NK cell function and SMAD4 protein expression were investigated.
Cytotoxicity of NK cells in patients with gastric cancer was inhibited before surgery, but it was enhanced in patients who were anesthetized by propofol compared with those who had sevoflurane. co-culture with BGC-823 cells significantly inhibited the cytotoxicity of NK cells, which was abolished by treatment of propofol or transforming growth factor (TGF)-β1. SMAD4 protein expression in the NK cell nucleus was significantly downregulated by TGF-β1 treatment and BGC-823 supernatant co-culture, and this expression could be restored by propofol.
Cytotoxicity of NK cells in patients with gastric cancer is low, but it can be promoted by propofol. Propofol regulates cytotoxicity in NK cells by promoting SMAD4, thereby affecting cellular function.
本研究旨在探讨丙泊酚和七氟醚对胃癌患者自然杀伤（NK）细胞细胞毒性的影响。
胃癌患者分别接受丙泊酚或七氟醚麻醉。分离外周血NK细胞并与BGC - 823胃癌细胞培养上清液共培养，采用流式细胞术分析细胞凋亡率和效应分子。研究丙泊酚和七氟醚对NK细胞功能及SMAD4蛋白表达的影响。
胃癌患者术前NK细胞的细胞毒性受到抑制，但与接受七氟醚麻醉的患者相比，接受丙泊酚麻醉的患者NK细胞毒性增强。与BGC - 823细胞共培养显著抑制NK细胞的细胞毒性，丙泊酚或转化生长因子（TGF）-β1处理可消除这种抑制作用。TGF -β1处理和BGC - 823上清液共培养显著下调NK细胞核内SMAD4蛋白表达，丙泊酚可恢复该表达。
胃癌患者NK细胞的细胞毒性较低，但丙泊酚可促进其细胞毒性。丙泊酚通过促进SMAD4调节NK细胞的细胞毒性，从而影响细胞功能。
