Department of Physical Chemistry, University of Madras, Chennai, Tamil Nadu, India.
School of Chemistry, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
J Biomol Struct Dyn. 2021 Apr;39(6):2242-2256. doi: 10.1080/07391102.2020.1747547. Epub 2020 Apr 15.
Designing the effective metallodrugs with amphiphilic nature is an active approach for the biomedical applications such as chemotheraphy, bioimaging, drug carrier, etc. To elaborate this, some fluorescent emissive surfactant-ruthenium(II) complexes and its precursor ruthenium(II) complexes have been interacted with calf thymus DNA (CT-DNA) for understanding the biophysical impacts of head and tail parts of the metallosurfactants. Here, DNA binding studies were examined by UV-visible absorption, fluorescence, circular dichroism and viscosity measurements. The obtained results showed that surfactant-ruthenium(II) complexes effectively bind with CT-DNA through hydrophobic interactions dominated moderate intercalation, whereas precursor ruthenium(II) complexes interact CT-DNA through electrostatic interactions dominated moderate intercalation. Also, increase of hydrophobic alkyl amine chain length as well as size of the head group in surfactant-ruthenium(II) complexes increased the binding affinity with CT-DNA, in which tail group played a dominant role. Further investigations of antibacterial, hemolytic and anticancer activities showed that desired biological activities could be obtained by tuning the head and tail groups of the metallodrugs in near future.Communicated by Ramaswamy H. Sarma.
设计具有两亲性的有效金属药物是一种用于生物医学应用的活跃方法，如化疗、生物成像、药物载体等。为了详细说明这一点，一些荧光发光表面活性剂-钌（II）配合物及其前体钌（II）配合物已与小牛胸腺 DNA（CT-DNA）相互作用，以了解金属表面活性剂的头和尾部分的生物物理影响。在这里，通过紫外-可见吸收、荧光、圆二色性和粘度测量研究了 DNA 结合研究。得到的结果表明，表面活性剂-钌（II）配合物通过疏水相互作用有效地与 CT-DNA 结合，主要是通过中等嵌入，而前体钌（II）配合物通过静电相互作用与 CT-DNA 相互作用，主要是通过中等嵌入。此外，表面活性剂-钌（II）配合物中疏水烷基胺链长和头基的增加增加了与 CT-DNA 的结合亲和力，其中尾基起主要作用。进一步研究抗菌、溶血和抗癌活性表明，通过调整金属药物的头基和尾基，在不久的将来可以获得所需的生物活性。由 Ramaswamy H. Sarma 传达。
