Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China.
Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen University, Xiamen, China.
Int Immunopharmacol. 2020 Jun;83:106428. doi: 10.1016/j.intimp.2020.106428. Epub 2020 Mar 24.
The effect of anti-TP0136 antibodies on the progression of syphilis is poorly understood. This study aimed to investigate the effect of anti-TP0136 antibodies on the progression of lesions in an infected rabbit model. Intramuscular injection of rTP0136 into rabbits in the immunized group (n = 4) elicited high titers of anti-TP0136 antibodies, and rabbits were then challenged with 10T. pallidum per site along their back. Lesion development was observed, and the injection sites were biopsied for tp0574 mRNA and histological analyses every week until the wound healed. The rabbits in the control group were injected with normal saline instead of rTP0136. Viable T. pallidum in the challenged rabbits was assessed with rabbit infectivity tests. The lesions in the immunized group took longer to heal than those in the control group (42 d vs. 28 d, P < 0.001) and had markedly higher levels of total cellular infiltrates. The mRNA level of tp0574 in the immunized group was significantly higher than that in the control group (P < 0.05). Viable T. pallidum was detected in rabbit lymph nodes in both the immunized and control groups. Our study showed that high titers of anti-TP0136 antibodies promoted the infiltration of inflammatory cells into local lesions and intensified tissue damage, thus delaying wound healing, and had no protective effect on the occurrence of syphilis in the rabbit model.
抗-TP0136 抗体对梅毒进展的影响知之甚少。本研究旨在探讨抗-TP0136 抗体对感染兔模型病变进展的影响。在免疫组（n=4）的兔中肌内注射 rTP0136 可诱导高滴度的抗-TP0136 抗体，然后用 10T. pallidum 每处接种到兔背部。观察病变发展，并每周对注射部位进行 tp0574mRNA 和组织学分析，直到伤口愈合。对照组的兔注射生理盐水而不是 rTP0136。用兔传染性试验评估受挑战兔中的活梅毒螺旋体。免疫组的病变愈合时间长于对照组（42 天 vs. 28 天，P<0.001），总细胞浸润明显更高。免疫组的 tp0574mRNA 水平明显高于对照组（P<0.05）。在免疫组和对照组的兔淋巴结中均检测到活梅毒螺旋体。我们的研究表明，高滴度的抗-TP0136 抗体促进炎症细胞浸润到局部病变并加重组织损伤，从而延迟伤口愈合，对兔模型中梅毒的发生没有保护作用。
