Ramiro-Cortijo David, Calle María de la, Rodríguez-Rodríguez Pilar, Pablo Ángel L López de, López-Giménez María R, Aguilera Yolanda, Martín-Cabrejas María A, González María Del Carmen, Arribas Silvia M
Department of Physiology, Faculty of Medicine, Universidad Autónoma de Madrid; C/ Arzobispo Morcillo 2, 28029 Madrid, Spain.
Obstetrics and Gynecology Service, La Paz University Hospital; Paseo de la Castellana 261, 28046 Madrid, Spain.
Antioxidants (Basel). 2020 Mar 25;9(4):269. doi: 10.3390/antiox9040269.
Twin pregnancies are increasing due to the rise in mothers' childbearing age and have a higher risk of fetal growth restriction (FGR) and prematurity. Therefore, early prediction of these events is important. Our aim was to analyze in the first trimester of pregnancy a possible association between antioxidants, including melatonin, in maternal plasma and the development of fetal complications in twin pregnancies. A single-center, prospective, and observational study was performed in 104 twin-pregnant women. A blood sample was extracted between the 9th and the 11th week of gestation, and plasma was obtained. Antioxidants (thiols, reduced glutathione, phenolic compounds, catalase, superoxide dismutase) and oxidative damage biomarkers (carbonyl groups and malondialdehyde) were assessed by spectrophotometry, and global scores were calculated from these parameters (Antiox-S, Prooxy-S). Melatonin and cortisol were evaluated by a competitive immunoassay. In the first trimester of pregnancy, Antiox-S was significantly lower in women who developed FGR compared to those with normal fetal growth; plasma melatonin was significantly lower in women with preterm compared to those with full-term births and exhibited a positive correlation with birth weight. Maternal cortisol showed a negative correlation with birth weight. We conclude that, for twin gestations, maternal plasma antioxidant status and melatonin could be potential biomarkers to be included in algorithms to predict FGR and preterm labor.
由于母亲生育年龄的增加，双胎妊娠正在增多，且双胎妊娠发生胎儿生长受限（FGR）和早产的风险更高。因此，对这些情况进行早期预测很重要。我们的目的是在妊娠早期分析孕妇血浆中包括褪黑素在内的抗氧化剂与双胎妊娠胎儿并发症发生之间的可能关联。对104例双胎妊娠妇女进行了一项单中心、前瞻性观察研究。在妊娠第9至11周采集血样，获得血浆。通过分光光度法评估抗氧化剂（硫醇、还原型谷胱甘肽、酚类化合物、过氧化氢酶、超氧化物歧化酶）和氧化损伤生物标志物（羰基和丙二醛），并根据这些参数计算总体评分（抗氧化-S、促氧化-S）。通过竞争性免疫测定法评估褪黑素和皮质醇。在妊娠早期，发生FGR的妇女的抗氧化-S显著低于胎儿生长正常的妇女；早产妇女的血浆褪黑素显著低于足月分娩妇女，且与出生体重呈正相关。孕妇皮质醇与出生体重呈负相关。我们得出结论，对于双胎妊娠，孕妇血浆抗氧化状态和褪黑素可能是预测FGR和早产的算法中应纳入的潜在生物标志物。
