School of Bioinnovation and Bio-based Product Intelligence, Faculty of Science, Mahidol University, Rama VI Rd, Bangkok 10400, Thailand.
Thailand Center of Excellence in Physics, Ministry of Higher Education, Science, Research and Innovation, 328 Si Ayutthaya Road, Bangkok 10400, Thailand.
Cells. 2020 Mar 25;9(4):790. doi: 10.3390/cells9040790.
Blood contains a diverse cell population of low concentration hematopoietic as well as non-hematopoietic cells. The majority of such rare cells may be bone marrow-derived progenitor and stem cells. This paucity of circulating rare cells, in particular in the peripheral circulation, has led many to believe that bone marrow as well as other organ-related cell egress into the circulation is a response to pathological conditions. Little is known about this, though an increasing body of literature can be found suggesting commonness of certain rare cell types in the peripheral blood under physiological conditions. Thus, the isolation and detection of circulating rare cells appears to be merely a technological problem. Knowledge about rare cell types that may circulate the blood stream will help to advance the field of cell-based liquid biopsy by supporting inter-platform comparability, making use of biological correct cutoffs and "mining" new biomarkers and combinations thereof in clinical diagnosis and therapy. Therefore, this review intends to lay ground for a comprehensive analysis of the peripheral blood rare cell population given the necessity to target a broader range of cell types for improved biomarker performance in cell-based liquid biopsy.
血液中含有多种低浓度的造血细胞和非造血细胞。这些稀有细胞大多数可能是骨髓来源的祖细胞和干细胞。由于外周循环中循环稀有细胞的数量稀少，尤其是在循环中，许多人认为骨髓以及其他器官相关的细胞进入循环是对病理状况的反应。尽管越来越多的文献表明，在生理条件下，外周血中某些稀有细胞类型很常见，但对此知之甚少。因此，循环稀有细胞的分离和检测似乎只是一个技术问题。对可能在血液中循环的稀有细胞类型的了解将有助于通过支持平台间的可比性、利用生物学上正确的截止值以及在临床诊断和治疗中“挖掘”新的生物标志物及其组合，来推进基于细胞的液体活检领域。因此，鉴于需要针对更广泛的细胞类型，以提高基于细胞的液体活检中生物标志物的性能，本综述旨在为外周血稀有细胞群的综合分析奠定基础。
