黄酮类化合物对在……中表达的神经氨酸酶的抑制作用 。 你提供的原文似乎不完整，“in.”后面应该还有具体的内容。
Inhibition effect of flavonoid compounds against neuraminidase expressed in .
作者信息
Nguyen Thi Thanh Hanh, Kang Hee-Kyoung, Kim Young-Min, Jang Tae-Su, Kim Doman
机构信息
1Department of Biotechnology and Bioengineering, and Research Institute for Catalysis, College of Engineering, Chonnam National University, Gwangju, 500-757 Korea.
2Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk, 580-185 Korea.
出版信息
Biotechnol Bioprocess Eng. 2014;19(1):70-75. doi: 10.1007/s12257-013-0599-3. Epub 2014 Mar 11.
Neuraminidase (NA) is one of the two glycoproteins on the surface of influenza virus, which cleaves terminal sialic acid residues and facilitates the release of virions from infected cells. The recombinant NA from H5N1 influenza virus strain A/Vietnam/1203/04 was expressed in X33 as a 45 kDa protein that displayed a of 9.96 ± 1.26 μM with fluorogenic substrate, 2'-(4-methylumbelliferyl)-α-D--acetyl neuraminic acid. Partially purified NA was used for the inhibition and kinetic assays with eight flavonoid compounds and gallic acid. Among them, gallocatechin gallate (GCG) showed the best inhibition against NA with the IC of 8.98 ± 0.46 μM and showed a competitive inhibition pattern with value of 8.34 ± 0.25 μM. In molecular docking experiments, GCG displayed a binding energy of -13.71 kcal/mol to the active site of NA and the galloyl moiety was required for NA inhibition activity.
神经氨酸酶（NA）是流感病毒表面的两种糖蛋白之一，它能切割末端唾液酸残基，促进病毒粒子从受感染细胞中释放。来自H5N1流感病毒株A/越南/1203/04的重组NA在X33中表达为一种45 kDa的蛋白质，其对荧光底物2'-(4-甲基伞形酮基)-α-D-N-乙酰神经氨酸的Km值为9.96±1.26μM。部分纯化的NA用于与八种黄酮类化合物和没食子酸进行抑制和动力学测定。其中，表没食子儿茶素没食子酸酯（GCG）对NA的抑制作用最佳，IC50为8.98±0.46μM，呈竞争性抑制模式，Ki值为8.34±0.25μM。在分子对接实验中，GCG与NA活性位点的结合能为-13.71 kcal/mol，NA抑制活性需要没食子酰基部分。
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