Zarytova V F, Zinov'ev V V, Ismagilov Z R, Levina A S, Repkova M N, Shikina N V, Evdokimov A A, Belanov E F, Balakhnin S M, Serova O A, Baiborodin S I, Malygin E G, Zagrebel'nyi S N
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 630090 Russia.
The Vektor State Research Center of Virology and Biotechnology, Federal Service for Supervision in the Sphere of Protecting Consumer Rights and Human Wellbeing (Federal State Research Institution), ABK, korp. 12, Kol'tsovo, Novosibirsk oblast, 630559 Russia.
Nanotechnol Russ. 2009;4(9):732. doi: 10.1134/S1995078009090158. Epub 2009 Oct 20.
In this study we examine the possibility that TiO nanoparticles and their conjugates can penetrate into cultivated cells without any special transfection procedures. Oligonucleotides and their derivates were conjugated with the TiO nanoparticles, which were obtained as colloidal solutions at a concentration of TiO 0.3M by TiCl hydrolysis. The electronic microscopy of various cell cultures (KCT, Vero, and MDCK) treated with nanoparticle solutions (20 µg/µl) showed that nanoparticles could enter the cells and accumulate in the vacuoles and phagosomes and form inclusions in cytoplasm. Thus, we demonstrated the penetration of TiO nanoparticles and their oligonucleotide conjugates into intracellular space without any auxiliary operations. Most other researches used electroporation techniques for similar purposes [1, 2, 5].
在本研究中，我们考察了二氧化钛纳米颗粒及其缀合物无需任何特殊转染程序即可穿透培养细胞的可能性。寡核苷酸及其衍生物与二氧化钛纳米颗粒缀合，通过TiCl水解以0.3M二氧化钛的浓度获得胶体溶液形式的纳米颗粒。用纳米颗粒溶液（20μg/μl）处理的各种细胞培养物（KCT、Vero和MDCK）的电子显微镜观察表明，纳米颗粒可进入细胞并积聚在液泡和吞噬体中，并在细胞质中形成内含物。因此，我们证明了二氧化钛纳米颗粒及其寡核苷酸缀合物无需任何辅助操作即可穿透细胞内空间。大多数其他研究为此类目的使用了电穿孔技术[1,2,5]。
