Levina A S, Repkova M N, Ismagilov Z R, Shikina N V, Mazurkova N A, Zarytova V F
Bioorg Khim. 2014 Mar-Apr;40(2):196-202. doi: 10.1134/s1068162014020095.
Antiviral activity of TiO2 * PL * DNA nanobiocomposites was studied on the MDCK cell culture infected with influenza A virus (subtype H3N2). DNA fragments in the nanocomposites are electrostatically bound to titanium dioxide nanoparticles pre-covered with polylysine. It was shown that TiO2 * PL * DNA(v3') nanocomposite bearing the DNA(v3') fragment targeted to the 3'-end of the noncoding region of segment 5 of viral RNA specifically inhibited the virus reproduction with the efficiency of 99.8 and 99.9% (or by factors of400 and 1000) at a low concentration of DNA(v3') in nanocomposite (0.1 and 0.2 µM, respectively). The TiO2 * PL * DNA(r) nanocomposite containing oligonucleotide noncomplementary to viral RNA or the oligonucleotide unbound to the nanoparticles show very low antiviral activity (inhibition by factors of3.5 and 1.3, respectively).
研究了TiO₂PLDNA纳米生物复合材料对感染甲型流感病毒（H3N2亚型）的MDCK细胞培养物的抗病毒活性。纳米复合材料中的DNA片段通过静电作用与预先覆盖有聚赖氨酸的二氧化钛纳米颗粒结合。结果表明，携带靶向病毒RNA第5节段非编码区3'端的DNA(v3')片段的TiO₂PLDNA(v3')纳米复合材料在纳米复合材料中DNA(v3')浓度较低（分别为0.1和0.2 μM）时，能特异性抑制病毒繁殖，抑制效率分别为99.8%和99.9%（或约为400倍和1000倍）。含有与病毒RNA非互补的寡核苷酸或未与纳米颗粒结合的寡核苷酸的TiO₂PLDNA(r)纳米复合材料显示出非常低的抗病毒活性（分别为约3.5倍和1.3倍的抑制）。
