埃及人群中甲型H1N1流感病毒感染与TNF - 308、IL6和IL8基因多态性：一项病例对照研究。
Influenza A (H1N1) virus infection and TNF-308, IL6, and IL8 polymorphisms in Egyptian population: a case-control study.
作者信息
Elsayed Shaimaa Moustafa, Hassanein Omayma Mohamed, Hassan Nagwa Hassan Ali
机构信息
1Molecular Biology & Epigenetics, Faculty of Medicine, Medical Ain Shams Research institute (MASRI), Ain Shams University Hospitals, Cairo, Egypt.
2Molecular Biology Department, Faculty of Medicine, Ain Shams Research Institute (MASRI), Ain Shams University Hospitals, Cairo, Egypt.
出版信息
J Basic Appl Zool. 2019;80(1):61. doi: 10.1186/s41936-019-0131-1. Epub 2019 Nov 21.
BACKGROUND
The importance of influenza is increasing mainly because of the appearance of novel pandemic strains such as swine and avian. Each year, influenza has spread around the world causing about 250,000-500,000 deaths and more than 5 million cases of severe illness.The objective is as follows: evaluating the outcomes of patients with influenza A (H1N1) virus in relation to certain TNF-308, IL6, and IL8 polymorphisms and identifying the associated factors with the severe outcome.
SUBJECT AND METHODS
This is a case-control study. The cases were patients confirmed by real-time polymerase chain reaction (RT-PCR) to be influenza A (H1N1) virus infected. The controls were healthy individuals. Medical history and outcome of the disease was registered. In all study participants, polymorphisms of TNF rs1800629, IL6 rs18138879, and IL8 rs4073; odds ratio (OR); and the 95% confidence interval (95% CI) were calculated.
RESULTS
Infection with influenza A (H1N1) virus was associated more with the following genotypes: TNF-308 AA (OR = 4.041; 95% CI = 1.215-13.4) and IL8 AA (OR = 3.273; 95% CI = 1.372-7.805). According to our study results, HCV (OR = 3.2, 95% CI 1.2-8.5), renal disease (OR = 3.4, 95% CI 0.9-13.6), cancer (OR = 3.1, 95% CI 0.3-31.1), TB (OR = 8.4, 95% CI 1.8-39.7), ICU (OR = 2.9, 95%1.2-7.1), and mortality (OR = 7.9, 95% CI 0.9-67.4) are considered as risk factors for influenza A (H1N1)-infected patients.
CONCLUSIONS
Our findings concluded that TNF-308 (AA) and IL8 (AA) polymorphisms may increase the susceptibility to be infected with H1N1influenza virus.
背景
流感的重要性日益增加，主要是因为出现了新型大流行毒株，如猪流感和禽流感毒株。每年，流感在全球传播，导致约25万至50万人死亡，以及超过500万例重症病例。目的如下：评估甲型H1N1流感病毒患者与某些肿瘤坏死因子-308（TNF-308）、白细胞介素6（IL6）和白细胞介素8（IL8）基因多态性相关的结局，并确定与严重结局相关的因素。
对象与方法
这是一项病例对照研究。病例为经实时聚合酶链反应（RT-PCR）确诊感染甲型H1N1流感病毒的患者。对照为健康个体。记录疾病病史和结局。计算所有研究参与者中肿瘤坏死因子rs1800629、白细胞介素6 rs18138879和白细胞介素8 rs4073的基因多态性、比值比（OR）以及95%置信区间（95%CI）。
结果
甲型H1N1流感病毒感染与以下基因型的关联更为密切：TNF-308 AA（OR = 4.041；95%CI = 1.215 - 13.4）和IL8 AA（OR = 3.273；95%CI = 1.372 - 7.805）。根据我们的研究结果，丙型肝炎病毒（HCV，OR = 3.2，95%CI 1.2 - 8.5）、肾脏疾病（OR = 3.4，95%CI 0.9 - 13.6）、癌症（OR = 3.1，95%CI 0.3 - 31.1）、结核病（TB，OR = 8.4，95%CI 1.8 - 39.7）、重症监护病房（ICU，OR = 2.9，95%CI 1.2 - 7.1）和死亡率（OR = 7.9，95%CI 0.9 - 67.4）被视为甲型H1N1流感病毒感染患者的危险因素。
结论
我们的研究结果表明，TNF-308（AA）和IL8（AA）基因多态性可能会增加感染H1N1流感病毒的易感性。
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