State Research Center of Virology and Biotechnology "Vector", Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB "Vector", Rospotrebnadzor), Koltsovo 630559, Russia.
Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia.
Int J Mol Sci. 2024 Jan 18;25(2):1156. doi: 10.3390/ijms25021156.
Tumor necrosis factor alpha (TNF-α) is a cytokine that is responsible for many processes associated with immune response and inflammation. It is involved in the development of an antiviral response to many virus infections. This factor was shown to be activated in influenza A virus infection, which enhances production of other cytokines. The overexpression of these cytokines can lead to a cytokine storm. To study the role of TNF-α in the development of pathologies associated with viral infection, we generated a knockout mouse strain. We demonstrated that these mice were characterized by a significant increase in the number of viral genomes compared to that in the parental strain, but the amount of live virus did not differ. A histopathology of the lungs in the genetically modified animals was significantly lower in terms of interalveolar septal infiltration. The generated model may be used to further study pathological processes in viral infections.
肿瘤坏死因子-α（TNF-α）是一种细胞因子，负责与免疫反应和炎症相关的许多过程。它参与了针对许多病毒感染的抗病毒反应的发展。已经表明该因子在甲型流感病毒感染中被激活，这增强了其他细胞因子的产生。这些细胞因子的过度表达可能导致细胞因子风暴。为了研究 TNF-α在与病毒感染相关的病理学发展中的作用，我们生成了一种 TNF-α 基因敲除小鼠品系。我们证明，与亲本株相比，这些小鼠的病毒基因组数量显著增加，但活病毒的数量没有差异。遗传修饰动物的肺组织病理学在肺泡间隔浸润方面显著降低。所生成的模型可用于进一步研究病毒感染中的病理过程。
